|This article needs additional citations for verification. (October 2008)|
|Isolated male patient diagnosed with Crimean-Congo hemorrhagic fever (C-CHF)|
|Group:||Group V ((-)ssRNA)|
Bunyaviridae is a family of negative-stranded, enveloped RNA viruses. Though generally found in arthropods or rodents, certain viruses in this family occasionally infect humans. Some of them also infect plants.
Bunyaviridae are vector-borne viruses. With the exception of Hantaviruses, transmission occurs via an arthropod vector (mosquitos, tick, or sandfly). Hantaviruses are transmitted through contact with deer mice feces. Incidence of infection is closely linked to vector activity, for example, mosquito-borne viruses are more common in the summer.
Human infections with certain Bunyaviridae, such as Crimean-Congo hemorrhagic fever virus, are associated with high levels of morbidity and mortality, consequently handling of these viruses must occur with a Biosafety level 4 laboratory. They are also the cause of severe fever with thrombocytopenia syndrome.
Hanta virus or Hantavirus Hemorrhagic fever, common in Korea, Scandinavia, Russia, and western North America, is associated with high fever, lung edema and pulmonary failure. Mortality is around 55%.
The antibody reaction plays an important role in decreasing levels of viremia.
There are currently ~330 viruses recognised in this family.
The family Bunyaviridae contains the genera:
- Genus Hantavirus; type species: Hantaan virus
- Genus Nairovirus; type species: Dugbe virus
- Genus Orthobunyavirus; type species: Bunyamwera virus
- Genus Phlebovirus; type species: Rift Valley fever virus
- Genus Tospovirus; type species: Tomato spotted wilt virus
Bunyaviridae have tripartite genomes consisting of a large (L), medium (M), and small (S) RNA segment. These RNA segments are single-stranded, and exist in a helical formation within the virion. Besides, they exhibit a pseudo-circular structure due to each segment's complementary ends. The L segment encodes the RNA Dependent RNA-polymerase, necessary for viral RNA replication and mRNA synthesis. The M segment encodes the viral glycoproteins, which project from the viral surface and aid the virus in attaching to and entering the host cell. The S segment encodes the nucleocapsid protein (N).
The L and M segment are negative sense. For the Genera of Phlebovirus and Tospovirus, the S segment is ambisense. Ambisense means that some of the genes on the RNA strand are negative sense and others are positive sense. The S segment codes for the viral nucleoprotein (N) in the negative sense and a nonstructural (NSs) protein in ambisense.
This ambisense arrangement requires two rounds of transcription to be carried out. First the negative sense RNA is transcribed to produce mRNA and a full length replicative intermediate. From this intermediate a subgenomic mRNA encoding the small segment nonstructural protein is produced while the polymerase produced following the first round of transcription can now replicate the full length RNA to produce viral genomes.
Bunyavirus RNA replicates in the cytoplasm, while the viral proteins transit through the ER and Golgi apparatus. Mature virions bud from the Golgi apparatus into vesicles which are transported to the cell surface.
No human vaccine is currently licensed for use in the U.S. Although animal vaccines against this virus are available, the uses of these vaccines are limited because of deleterious effects or incomplete protection, justifying further studies to improve the existing vaccines or to develop others.
- Plyusnin, A; Elliott, RM (editor) (2011). Bunyaviridae: Molecular and Cellular Biology. Caister Academic Press. ISBN 978-1-904455-90-5.
- "00.011. Bunyaviridae". ICTVdB - The Universal Virus Database, version 4. 2006. Retrieved 2009-01-01.
- Viralzone: Bunyaviridae
- ICTVdb Index of Viruses - Bunyaviridae
- The Big Picture Book of Viruses: Bunyaviridae
- Bunyaviridae Genomes — database search results from the Viral Bioinformatics Resource Center
- Virus Pathogen Database and Analysis Resource (ViPR): Bunyaviridae