CARD11

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CARD11
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesCARD11, BENTA, BIMP3, CARMA1, IMD11, PPBL, caspase recruitment domain family member 11, IMD11A
External IDsOMIM: 607210 MGI: 1916978 HomoloGene: 13024 GeneCards: CARD11
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_032415
NM_001324281

NM_175362

RefSeq (protein)

NP_001311210
NP_115791

NP_780571

Location (UCSC)Chr 7: 2.91 – 3.04 MbChr 5: 140.86 – 140.99 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Caspase recruitment domain-containing protein 11 also known as CARD-containing MAGUK protein 1 (Carma 1) is a protein in the CARD-CC protein family that in humans is encoded by the CARD11 gene.[5][6][7] CARD 11 is a membrane associated protein that is found in various human tissues, including the thymus, spleen, liver, and peripheral blood leukocytes. Similarly, CARD 11 is also found in abundance in various lines of cancer cells.[5]


Function[edit]

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). CARD11 (CARMA1) has a domain structure similar to that of CARD10 (CARMA3) and CARD14 (CARMA2) as a member of the CARD-CC family with a C terminal MAGUK domain (the so-called CARMA proteins). The CARD domain of proteins in the CARD-CC family have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of NF-κB activation by recruitment and activation of MALT1. When overexpressed in cells, this protein family activates NF-κB and induces the phosphorylation of BCL10.[7]

CARD11 is critical for T cell and B cell function and is activated after T cell receptor or B cell receptor stimulation. After receptor stimulation, CARD11 is phosphorylated by PKC-θ (in T cells) or PKC-β (in B cells). The phosphorylation induces formation of filamentous CARD11 multimers that recruit BCL10 and MALT1, which in turn activates NF-κB. Loss of function mutations in CARD11 cause severe combined immunodeficiency (SCID) since the function of cells critical for adaptive immunity are disrupted.

Structure[edit]

This is the predicted structure of CARD11 produced by AlphaFold. The different colored regions reflect the confidence that a particular residue is in that location, with dark blue being the most confident and orange indicating the least confidence.

The structure of CARD11 involves multiple domains that impact the protein's ability to activate BCL10 and NF-κB activity. CARD11 has a CARD domain, a serine-threonine rich region, is associated with the N-terminus, which is essential for NF-κB signaling activity. The region following the CARD domain is highly coiled. In deleting the CARD domain, all NF-κB signaling activity was prevented. The CARD domain on CARD11 interacts with the CARD domain on BCL10 to initiate the signaling pathway.[5]

On the C-terminus of CARD11 there is the MAGUK domain that is associated with the cell membrane. This domain is often referred to as the inhibitory domain. Protein kinase C activates CARD11 by phosphorylating serine residues within the inhibitory domain.[5][8]

Interactions[edit]

CARD11 has been shown to interact with BCL10.[9] This interaction occurs between the CARD domain on BCL10 and the CARD domain on CARD11, and results in signal propagation and NF-κB activation.[8]

References[edit]

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000198286 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000036526 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ a b c d Bertin J, Wang L, Guo Y, Jacobson MD, Poyet JL, Srinivasula SM, Merriam S, DiStefano PS, Alnemri ES (Apr 2001). "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B". J. Biol. Chem. 276 (15): 11877–82. doi:10.1074/jbc.M010512200. PMID 11278692. S2CID 35815019.
  6. ^ Gaide O, Martinon F, Micheau O, Bonnet D, Thome M, Tschopp J (May 2001). "Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation". FEBS Lett. 496 (2–3): 121–7. doi:10.1016/S0014-5793(01)02414-0. PMID 11356195. S2CID 22024213.
  7. ^ a b "Entrez Gene: CARD11 caspase recruitment domain family, member 11".
  8. ^ a b Holliday MJ, Witt A, Rodriguez Gama A, Walters B, Arthur C, Halfman R, Rohou A, Dueber E, Fairbrother W (2019). "Structures of autoinhibited and polymerized forms of CARD9 reveal mechanisms of CARD9 and CARD11 activation". Nat Commun. 10 (3070): 3070. Bibcode:2019NatCo..10.3070H. doi:10.1038/s41467-019-10953-z. PMC 6624267. PMID 31296852.
  9. ^ Bertin J, Wang L, Guo Y, Jacobson MD, Poyet JL, Srinivasula SM, Merriam S, DiStefano PS, Alnemri ES (April 2001). "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B". J. Biol. Chem. 276 (15): 11877–82. doi:10.1074/jbc.M010512200. PMID 11278692. S2CID 35815019.

External links[edit]

Further reading[edit]