|Description||Protein Structure Classification|
|Research center||University College London|
|Laboratory||Institute of Structural and Molecular Biology|
|Primary citation||Cuff & al. (2011)|
The CATH Protein Structure Classification is a semi-automatic, hierarchical classification of protein domains published in 1997 by Christine Orengo, Janet Thornton and their colleagues. CATH shares many broad features with its principal rival, SCOP, however there are also many areas in which the detailed classification differs greatly.
The name CATH is an acronym of the four main levels in the classification.
|1||Class||the overall secondary-structure content of the domain|
|2||Architecture||high structural similarity but no evidence of homology. Equivalent to a fold in SCOP|
|3||Topology||a large-scale grouping of topologies which share particular structural features|
|4||Homologous superfamily||indicative of a demonstrable evolutionary relationship. Equivalent to the superfamily level of SCOP.|
In order to better understand the CATH classification system it is useful to know how it is constructed: much of the work is done by automatic methods, however there are important manual elements to the classification.
The very first step is to separate the proteins into domains. It is difficult to produce an unequivocal definition of a domain and this is one area in which CATH and SCOP differ.
The domains are automatically sorted into classes and clustered on the basis of sequence similarities. These groups form the H levels of the classification. The topology level is formed by structural comparisons of the homologous groups. Finally, the Architecture level is assigned manually.
Class Level classification is done on the basis of 4 criteria:
- Secondary structure content;
- Secondary structure contacts;
- Secondary structure alternation score; and
- Percentage of parallel strands.
- Cuff, Alison L; Sillitoe Ian, Lewis Tony, Clegg Andrew B, Rentzsch Robert, Furnham Nicholas, Pellegrini-Calace Marialuisa, Jones David, Thornton Janet, Orengo Christine A (Jan 2011). "Extending CATH: increasing coverage of the protein structure universe and linking structure with function". Nucleic Acids Res. (in eng) (England) 39 (Database issue): D420–6. doi:10.1093/nar/gkq1001. PMC 3013636. PMID 21097779.
- Orengo CA, Michie AD, Jones S, Jones DT, Swindells MB, Thornton JM (1997). "CATH--a hierarchic classification of protein domain structures". Structure 5 (8): 1093–1108. doi:10.1016/S0969-2126(97)00260-8. PMID 9309224.
- Hadley C, Jones DT (1999). "A systematic comparison of protein structure classifications: SCOP, CATH and FSSP". Structure 7 (9): 1099–1112. doi:10.1016/S0969-2126(99)80177-4. PMID 10508779.
- Day R, Beck DA, Armen RS, Daggett V (2003). "A consensus view of fold space: Combining SCOP, CATH, and the Dali Domain Dictionary". Protein Sci. 12 (10): 2150–2160. doi:10.1110/ps.0306803. PMC 2366924. PMID 14500873.