CDC34 is a gene encoding a protein product that has ubiquitin conjugating activity. CDC34 was originally discovered by work in baker's yeast as a gene that has a role in the cell division cycle. Cdc34 in yeast targets numerous substrates (Sic1, Far1, Cln1, Cln2) for ubiquitin mediated degradation. Ubiquitin-conjugating enzyme E2 R1 is a protein that in humans is encoded by the CDC34gene.
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Gonen H, Bercovich B, Orian A et al. (1999). "Identification of the ubiquitin carrier proteins, E2s, involved in signal-induced conjugation and subsequent degradation of IkappaBalpha.". J. Biol. Chem.274 (21): 14823–30. doi:10.1074/jbc.274.21.14823. PMID10329681.CS1 maint: Explicit use of et al. (link)
Reymond F, Wirbelauer C, Krek W (2000). "Association of human ubiquitin-conjugating enzyme CDC34 with the mitotic spindle in anaphase.". J. Cell. Sci.113 (10): 1687–94. PMID10769200.
Charrasse S, Carena I, Brondani V et al. (2000). "Degradation of B-Myb by ubiquitin-mediated proteolysis: involvement of the Cdc34-SCF(p45Skp2) pathway.". Oncogene19 (26): 2986–95. doi:10.1038/sj.onc.1203618. PMID10871850.CS1 maint: Explicit use of et al. (link)
Strack P, Caligiuri M, Pelletier M et al. (2000). "SCF(beta-TRCP) and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4.". Oncogene19 (31): 3529–36. doi:10.1038/sj.onc.1203647. PMID10918611.CS1 maint: Explicit use of et al. (link)
Duan W, Sun B, Li TW et al. (2001). "Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo.". Gene256 (1-2): 113–21. doi:10.1016/S0378-1119(00)00365-6. PMID11054541.CS1 maint: Explicit use of et al. (link)
Block K, Boyer TG, Yew PR (2001). "Phosphorylation of the human ubiquitin-conjugating enzyme, CDC34, by casein kinase 2.". J. Biol. Chem.276 (44): 41049–58. doi:10.1074/jbc.M106453200. PMID11546811.
Wu K, Chen A, Tan P, Pan ZQ (2002). "The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34.". J. Biol. Chem.277 (1): 516–27. doi:10.1074/jbc.M108008200. PMID11675391.
Semplici F, Meggio F, Pinna LA, Oliviero S (2002). "CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation.". Oncogene21 (25): 3978–87. doi:10.1038/sj.onc.1205574. PMID12037680.