CELA3B

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Chymotrypsin-like elastase family, member 3B
Identifiers
Symbols CELA3B ; CBPP; E1; EL-1; ELA3B
External IDs MGI1915118 HomoloGene128227 ChEMBL: 4995 GeneCards: CELA3B Gene
EC number 3.4.21.70
Orthologs
Species Human Mouse
Entrez 23436 67868
Ensembl ENSG00000219073 ENSMUSG00000023433
UniProt P08861 Q9CQ52
RefSeq (mRNA) NM_007352 NM_026419
RefSeq (protein) NP_031378 NP_080695
Location (UCSC) Chr 1:
22.3 – 22.33 Mb
Chr 4:
137.42 – 137.43 Mb
PubMed search [2] [3]

Chymotrypsin-like elastase family member 3B also known as elastase-3B, protease E, or fecal elastase is an enzyme that in humans is encoded by the CELA3B gene.[1][2][3]

Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B (this protein).[4]

Function[edit]

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.[3]

Clinical significance[edit]

Fecal elastase is a medical test that measures how well the pancreas is functioning.

The fecal elastase test measures the concentration of the elastase-3B enzyme found in fecal matter with an enzyme-linked immunosorbent assay (ELISA). Results of this test can give a good indication of exocrine pancreatic status, and the test is less invasive and expensive than the current "gold standard", secretin-cholecystokinin test.[5] Levels of fecal elastase lower than 200 μg / g of stool indicate an exocrine insufficiency. Correlations between low levels and chronic pancreatitis[6] and cancer[7] have been reported.

References[edit]

  1. ^ Tani T, Ohsumi J, Mita K, Takiguchi Y (Feb 1988). "Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning". J Biol Chem 263 (3): 1231–9. PMID 2826474. 
  2. ^ Shirasu Y, Takemura K, Yoshida H, Sato Y, Iijima H, Shimada Y, Mikayama T, Ozawa T, Ikeda N, Ishida A, et al. (Dec 1988). "Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes". J Biochem 104 (2): 259–64. PMID 2460440. 
  3. ^ a b "Entrez Gene: ELA3B elastase 3B, pancreatic". 
  4. ^ "Entrez Gene: chymotrypsin-like elastase family, member 1". 
  5. ^ Molinari I. et al. (September 2004). "Fecal chymotrypsin and elastase-1 determination on one single stool collected at random: diagnostic value for exocrine pancreatic status". Clin Biochem 37 (9): 758–763. doi:10.1016/j.clinbiochem.2004.03.010. PMID 15329313. 
  6. ^ Fecal Elastase 1 ELISA For Exocrine Pancreatic Insufficiency: Comparison With ERCP-Morphology And Fecal Fat Excretion [1]
  7. ^ Role of Fecal Elastase 1 in Pancreatic Cancer: A Pilot Study

Further reading[edit]