CENPH

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Centromere protein H
Identifiers
Symbols CENPH ; NNF1; PMF1
External IDs OMIM605607 MGI1349448 HomoloGene32519 GeneCards: CENPH Gene
Orthologs
Species Human Mouse
Entrez 64946 26886
Ensembl ENSG00000153044 ENSMUSG00000045273
UniProt Q9H3R5 Q9QYM8
RefSeq (mRNA) NM_022909 NM_021886
RefSeq (protein) NP_075060 NP_068686
Location (UCSC) Chr 5:
68.49 – 68.51 Mb
Chr 13:
100.76 – 100.78 Mb
PubMed search [1] [2]
CENP-H
Identifiers
Symbol CENP-H
Pfam PF05837
InterPro IPR008426

Centromere protein H is a protein that in humans is encoded by the CENPH gene.[1][2][3]

Function[edit]

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. CENP-H is required for the localisation of CENP-C, but not CENP-A, to the centromere. However, it may be involved in the incorporation of newly synthesised CENP-A into centromeres via its interaction with the CENP-A/CENP-HI complex.[4] CENP-H localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.[5] CENP-H contains a coiled-coil structure and a nuclear localisation signal.[5]

Studies show that CENP-H may be associated with certain human cancers.[6][7]

CENP-H shows sequence similarity to the Schizosaccharomyces pombe kinetochore protein Fta3 which is a subunit of the Sim4 complex. This complex is required for loading the DASH complex onto the kinetochore via interaction with dad1. Fta2, Fta3 and Fta4 associate with the central core and inner repeat region of the centromere.[8]

Other Protein Interactions[edit]

CENPH has also been shown to interact with KIAA0090.[9] The significance of this interaction is unclear.

References[edit]

  1. ^ Sugata N, Li S, Earnshaw WC, Yen TJ, Yoda K, Masumoto H, Munekata E, Warburton PE, Todokoro K (Jan 2001). "Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere--kinetochore complexes". Hum Mol Genet 9 (19): 2919–26. doi:10.1093/hmg/9.19.2919. PMID 11092768. 
  2. ^ Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nat Cell Biol 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821. 
  3. ^ "Entrez Gene: CENPH centromere protein H". 
  4. ^ Fukagawa, T.; Mikami, Y.; Nishihashi, A.; Regnier, V.; Haraguchi, T.; Hiraoka, Y.; Sugata, N.; Todokoro, K.; Brown, W.; Ikemura, T. (2001). "CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells". The EMBO Journal 20 (16): 4603–4617. doi:10.1093/emboj/20.16.4603. PMC 125570. PMID 11500386.  edit
  5. ^ a b Sugata N, Munekata E, Todokoro K (September 1999). "Characterization of a novel kinetochore protein, CENP-H". J. Biol. Chem. 274 (39): 27343–6. doi:10.1074/jbc.274.39.27343. PMID 10488063. 
  6. ^ Guo XZ, Zhang G, Wang JY, Liu WL, Wang F, Dong JQ, Xu LH, Cao JY, Song LB, Zeng MS (2008). "Prognostic relevance of Centromere protein H expression in esophageal carcinoma". BMC Cancer 8: 233. doi:10.1186/1471-2407-8-233. PMC 2535782. PMID 18700042. 
  7. ^ Liao WT, Song LB, Zhang HZ, Zhang X, Zhang L, Liu WL, Feng Y, Guo BH, Mai HQ, Cao SM, Li MZ, Qin HD, Zeng YX, Zeng MS (January 2007). "Centromere protein H is a novel prognostic marker for nasopharyngeal carcinoma progression and overall patient survival". Clin. Cancer Res. 13 (2 Pt 1): 508–14. doi:10.1158/1078-0432.CCR-06-1512. PMID 17255272. 
  8. ^ Liu X, McLeod I, Anderson S, Yates JR, He X (August 2005). "Molecular analysis of kinetochore architecture in fission yeast". EMBO J. 24 (16): 2919–30. doi:10.1038/sj.emboj.7600762. PMC 1187945. PMID 16079914. 
  9. ^ Prieto C, De Las Rivas J (July 2006). "APID: Agile Protein Interaction DataAnalyzer". Nucleic Acids Res. 34 (Web Server issue): W298–302. doi:10.1093/nar/gkl128. PMC 1538863. PMID 16845013. 

Further reading[edit]

This article incorporates text from the public domain Pfam and InterPro IPR008426