CHAPS detergent

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CHAPS detergent
Structural formula of CHAPS detergent
Identifiers
Abbreviations CHAPS
CAS number 75621-03-3 YesY
PubChem 107670 YesY, 24883538 (),(5R,7S,

16SYesY, 10371606 (),(1S,5R,11S,
16SYesY, 25244640 (4R),(2S,5R,7S,
15R,16S), 18397788 (4R),(2S,5R,
14R,15R,16SYesY, 9895216 (4S),
(2S,5R,14R,15S,16SYesY

ChemSpider 96843 YesY
KEGG C11321 YesY
MeSH 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate
ChEMBL CHEMBL450950 YesY
Jmol-3D images Image 1
Image 2
Properties
Molecular formula C32H58N2O7S
Molar mass 614.88 g mol−1
log P -4.32 (predicted)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 N (verify) (what is: YesY/N?)
Infobox references

CHAPS is a zwitterionic detergent used in the laboratory to solubilize biological macromolecules such as proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins, which are often sparingly soluble or insoluble in aqueous solution due to their native hydrophobicity. CHAPS can also be used in conjunction with nonionic detergents such as Triton X-100.

CHAPS is an abbreviation for 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. A related detergent, called CHAPSO, has the same basic chemical structure with an additional hydroxyl functional group; its full chemical name is 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate. Both detergents have low light absorbance in the ultraviolet region of the electromagnetic spectrum, which is useful for laboratory workers monitoring ongoing chemical reactions or protein-protein binding with UV/Vis spectroscopy. The CMC (Critical Micelle Concentration) of this compound is 8-10mM.

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