Calf-intestinal alkaline phosphatase

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Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands.[1][2] This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate. [3] This prevents recircularization of linearized vector DNA and improves the yield of vector containing the appropriate insert.

References[edit]

  1. ^ Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  2. ^ Seeburg, P.H. et al. (1977) Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone. Nature 270, 486–94
  3. ^ Ullrich, A. et al. (1977) Rat insulin genes: Construction of plasmids containing the coding sequences. Science 196, 1313–9.