Cap binding complex
Nuclear cap-binding complex
In the nucleus freshly transcribed mRNA molecules are bound on the 5' cap by the nuclear cap-binding complex of Cbc1/Cbc2 in yeast or CBC20/CBC80 in metazoans. These aid in the export of the mRNA and protect it from decapping. They also serve as a marker for the so-called pioneer round of translation when the message is examined by nonsense mediated decay.
Cytoplasmic cap-binding complex
After the first round of translation the nuclear complex is replaced by the translation initiation factors eIF-4E and eIF-4G. These regulate translation in response to the state of the cell via their phosphorylation state and again protect the message from decapping.
When translationally repressed or marked for decay by various mechanisms the 5' cap is bound by the mRNA decapping enzyme DCP2. A host of proteins accompany it including UPF1, UPF2, UPF3A, Dcp1, Dhh1, XRN1, and others. The decapping enzyme removes the 5' cap leading to destruction of the message.
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- nuclear cap-binding protein complex at the US National Library of Medicine Medical Subject Headings (MeSH)