|Group:||Group VII (dsDNA-RT)|
Viruses belonging to the Caulimoviridae family are termed DNA reverse-transcribing viruses (or pararetroviruses) i.e. viruses that contain a reverse transcription stage in their replication cycle. This family contains all plant viruses that consist of a double-stranded DNA genome that has a reverse transcribing phase in its lifecycle.
The following genera are included in this family:
- Genus Badnavirus; type species: Commelina yellow mottle virus
- Genus Caulimovirus; type species: Cauliflower mosaic virus
- Genus Cavemovirus; type species: Cassava vein mosaic virus
- Genus Florendovirus; type species: Vitis vinifera A virus
- Genus Petuvirus; type species: Petunia vein clearing virus
- Genus Rosadnavirus; type species: Rose yellow vein virus
- Genus Solendovirus; type species: Tobacco vein clearing virus
- Genus Soymovirus; type species: Soybean chlorotic mottle virus
- Genus Tungrovirus; type species: Rice tungro bacilliform virus
The Rose yellow vein virus has an unusual genome organisation that appears to be unique in this family.
Virus particle structure
All viruses of this family are unenveloped. Virus particles contain nucleocapsids one of two forms; either bacilliform or isometric. The type of nucleocapsid incorporated into the virus structure determines the size of the virus. Bacilliform nucleocapsid viruses are approximately 35-50 nm diameter and can be 900 nm in length. Isometric nucleocapsid viruses are on average 45-50 nm in diameter and show icosahdral symmetry.
Genome structure and replication
The genomes of viruses from this family contain monopartite, double-stranded DNA in either an open circular or linear structure. The size of the genome is usually between 6000-8000 base pairs. Depending on the virus, DNA can contain either one open reading frame (ORF) as observed in Petuviruses, or up to eight ORFs such as in the Soymoviruses. Proteins found to be encoded in this virus family genome include reverse transcriptase, proteases, nucleocapsids and transactivators - there are other proteins essential for replication that have yet to be assigned a specific function.
Replication takes place in both the cytoplasm and nucleus of host cells. Firstly, the viral genome enters the cytoplasm. The viral DNA forms supercoiled mini-chromosome structures upon entering the host nucleus. The viral DNA is transcribed into polyadenylated RNA which is terminally redundant (due to transcription occurring twice for some parts of the DNA). Newly transcribed RNA enters the cytoplasm once more where it has two roles. It can either be used as a template for viral protein synthesis, or it can undergo reverse transcription by viral encoded reverse transcriptase to make dsDNA. This DNA can then reenter the nucleus for amplification.
As replication requires the use of RNA intermediate, viruses from the Caulimoviridae family are not true dsDNA viruses - instead they are termed DNA reverse-transcribing viruses. As this property is also found in retroviruses, these families have been compared. However, there are several important differences between retroviruses and viruses from the Caulimoviridae family. Unlike retroviruses, they do not require the integration of viral genome into the host's in order to replicate and for this reason their genome does not encode the enzymatic protein integrase.
Although they do not regularly integrate into the host genome, sequences from certain caulimoviruses, such as Petunia vein clearing virus and Banana streak virus have been found in host genomes. These are termed Endogenous Pararetroviruses, or EPRVs.
- Notes on family; Caulimoviridae. http://www.dpvweb.net/notes/showfamily.php?family=Caulimoviridae
- Livia Stavolone, Antonio Ragozzino,and Thomas Hohn. Characterization of Cestrum yellow leaf curling virus: a new member of the family Caulimoviridae. Journal of General Virology 84 (2003), 3459-3464. http://vir.sgmjournals.org/cgi/content/full/84/12/3459
- 14th Swiss Plant Molecular and Cell Biology Conference, March 2005. http://www.unifr.ch/plantbio/skmb/abstracts.pdf
- Mollov D, Lockhart B, Zlesak DC, Olszewski N (2012) Complete nucleotide sequence of rose yellow vein virus, a member of the family Caulimoviridae having a novel genome organization. Arch Virol
- Viralzone: Caulimoviridae
- Spumavirus at the US National Library of Medicine Medical Subject Headings (MeSH)