Center for Applied Genomics
|Type||Genomics Research Center|
|Key people||Hakon Hakonarson, Director|
|Parent||Children's Hospital of Philadelphia|
The Center for Applied Genomics (CAG) is a Center of Emphasis at the Children's Hospital of Philadelphia with the primary goal of discovering and translating basic research findings into medical innovations.
The Center is one of the world’s largest genetics research and analytical facilities. It is the primary center at a pediatric hospital with access to state-of-the-art high-throughput genotyping technology, and has processed genetic samples from over 100,000 people.
The Center is focused on detecting the genetic causes of some of the most prevalent childhood diseases including (but not limited to) asthma, obesity, ADHD, autism, diabetes, inflammatory bowel disease, epilepsy, schizophrenia, and pediatric cancer, all of which are thought to involve multiple, interacting genes within the body.
The mission of the Center for Applied Genomics (CAG) is to develop new and better ways to diagnose and treat children affected by complex medical disorders. The Center is a specialized Center of Emphasis at the Children’s Hospital of Philadelphia with the primary goal of translating basic research findings to medical innovations.
We aim to discover genetic causes for the most prevalent diseases of childhood including asthma, autism, diabetes, epilepsy, obesity, schizophrenia, and pediatric cancer. Ultimately, our objective is to generate new diagnostic tests and to guide physicians to the most appropriate therapies.
The Center is one of the world's largest genetics research programs, and the lead center at a pediatric hospital to have large-scale access to state-of-the-art high-throughput genotyping technology.
Although highly heritable, genetic correlates of attention-deficit/hyperactivity disorder (ADHD) have been difficult to pinpoint. In 2009, CAG researchers identified copy number variants as a potential cause of the disorder. CNVs are relatively large segments of chromosomes where the DNA has been deleted, duplicated, or rearranged. The group found 222 CNVs that were more common in individuals with ADHD than in unrelated healthy individuals. These CNVs tended to concentrate in areas that had previously been associated with other neurodevelopmental disorders including autism, schizophrenia and Tourette syndrome. They also tended to occur at or near genes known to be important for learning, synapse transmission, and nervous system development. This paper was one of the first to pinpoint CNVs as a genetic cause of ADHD and was important in identifying previously unassociated genes.
Asthma is a complex disease with genetic and environmental causes. It affects more than 6% of children in the developed world (Fanta, 2009). Because of its complexity, candidate genes for asthma have been difficult to determine. In 2010, the Center published a genome-wide association study (GWAS) of 3,377 children with asthma and 5,579 healthy children. GWAS allows researchers to examine genetic variations across an individual’s entire genome, and compare variations between affected and unaffected groups. They discovered a region on chromosome 17 and a previously unassociated region on chromosome 1 that strongly correlated with susceptibility to asthma. The region in chromosome 1 was especially associated with asthma in the children of African ancestry, and contains a gene, DENND1B, that is expressed by natural killer cells – a critical component of the immune system. Targeting the DENND1B gene may be a promising avenue for future treatments of asthma.
Although twin studies suggest that ASDs are highly heritable, specific genes have been difficult to identify. In 2009, the Center conducted a genome-wide association study (GWAS) on a group of 780 families (3,101 individuals) with affected children, a second group of 1,204 affected individuals, and 6,491 controls, all of whom had European ancestry. GWAS allows us to examine an individual’s entire genome. By comparing genomics variations between the groups, CAG researchers led by Kai Wang identified six genetic markers between two specific genes that confirmed susceptibility to ASDs. This was the first study to demonstrate a genome-wide significant association of this kind. The two genes, cadherin 10 and cadherin 9 are sticky molecules that help neurons bind together. They play an important role in neurodevelopment, and may be important in helping us understand the neuropathology of autism.
In 2009, the Center published a second paper in the journal Nature that identified copy number variations (CNVs) as important genetic features in autism. Led by Joseph Glessner, the group examined 859 autism cases and 1,409 healthy children of European ancestry. The autism group had significantly more CNVs on or near genes that had previously been associated with the disorder. Further, they identified several new susceptibility genes in ubiquitin networks that were associated with the autism group. Ubiquitins are small proteins that help to destroy unneeded or damaged proteins. Damage to ubiquitin networks can theoretically cause neurological changes that may underlie autism and are an important avenue for future research into the disorder.
The Center for Applied Genomics has been at the forefront of research into the genetic causes of a number of cancers and has had a major impact on our understanding of how cancer occurs.
Neuroblastoma is a type of cancer most commonly found in children that affects the sympathetic nervous system (part of the nervous system that helps control our organs). It is often lethal. In 2008, the Center group collaborated with the Maris Lab at CHOP to publish the first of three important papers on the genetic causes of neuroblastoma. They performed a genome-wide association study (GWAS) comparing the genomes of 1032 patients and 2043 controls. The researchers found a significant association between neuroblastoma and a region of chromosome 6.
In 2009, the Center followed-up this finding with another GWAS that focused on a 397-person high-risk subset of the neuroblastoma group. They identified a region on chromosome 2 on or near the BARD1 gene, which has been found to regulate cell growth and tumor suppressants. These data show that a common variation in the BARD1 gene contributes to aggressive neuroblastoma – the most clinically important form of the disease.
In 2009, the Center contributed another study identifying copy number variations (CNVs) as a potential cause of neuroblastoma. CNVs are segments of DNA consisting of deleted, duplicated, or rearranged genetic material. They identified a CNV on chromosome 1 that associated with the disorder. It behaved similarly to a class of genes known as neuroblastoma breakpoint family (NBPF) genes and was thus implicated as a previously unknown member of the neuroblastoma breakpoint family gene. The study was the first germline CNV study in any cancer.
Testicular cancer is the most common form of cancer in men between the ages of 15 and 34, but also peaks in infancy and old age. In 2009, in collaboration with the Nathanson Lab at the University of Pennsylvania, the Center published the results of a genome-wide associated study that examined the genomes of 227 patients with testicular germ cell tumors and 919 controls. They identified an area on chromosome 5 as a major risk factor for the disease, which was in the region of a gene called SPRY4. A specific copy of this gene is associated with a 40% greater chance of testicular cancer. Even more strikingly, they identified a region in chromosome 12 within a gene called KITLG. Patients with a specific form of the KITLG gene were much more likely to have testicular cancer – each copy of the gene-form increased risk of testicular cancer threefold. This represents one of the largest effect sizes reported in cancer.
Crohn's disease (CD) is one of the two main forms of inflammatory bowel disease (the other being ulcerative colitis). It affects the gastrointestinal tract, and may cause pain, diarrhea, and vomiting, and can result in significant weight loss. The genetic associations of CD have remained elusive. In 2008, the Center pioneered an alternative strategy for examining the disorder by focusing on age-of-onset. To this end, they carried out a genome-wide association study of 1,011 individuals with pediatric-onset IBD and 4,250 matched controls. Researchers identified and replicated two previously unreported regions on chromosome 20 and chromosome 21 that predicted childhood IBD. These regions were located near the genes TNFRSF6B and PSMG1 respectively. The first of these genes is now thought to be intimately linked to the biology of IBD.
In a subsequent paper, the Center applied pathway analysis to focus on multiple regions in the genome that may interact to cause Crohn’s disease. Researchers led by Kai Wang identified an association between CD and a network of 20 genes. The network contains many interleukins – proteins that are critical components of the immune system. Interestingly, many of the genes in the pathway did not correlate significantly with CD independently. However, when analyzed together, the network of genes did significantly associate with the disorder. This finding represents an important proof-of-principle as the first demonstration of the power of a pathway approach to understanding human genomics.
In 2009, the Center also published a genome-wide association study of inflammatory bowel diseases (Crohn's disease and ulcerative colitis) in 3,426 affected individuals and 11,963 genetically matched controls. Researchers identified five new regions associated with early-onset IBD, and detected associations at a number of loci previously implicated in adult-onset IBD. This provides an important demonstration of the close genetic relationship between early- and adult-onset IBD.
Schizophrenia is a complex disorder that affects about a half percent of the world's population. Typically, the first symptoms of schizophrenia typically appear in adolescence. In a genome-wide association study of 1,735 schizophrenic patients and 3,485 healthy adults, the Center identified copy number variations as a potential cause of the disorder. In the schizophrenia group, researchers located CNVs near the CACNA1B and DOC2A genes, both of which facilitate calcium signals that are important to neurotransmission in the brain. They also identified CNVs near the RET and RIT2 genes, which are known to be involved in brain development. Interestingly some of the regions associated with schizophrenia have previously been found to associate with autism and ADHD. This provides a strong indication that many psychiatric disorders stem share similar neurodevelopmental features.
Type 1 Diabetes
Type 1 diabetes (T1D) in children results from autoimmune destruction of cells in the pancreas, leading to an insufficient production of insulin. It is fatal unless treated by insulin. In 2007, CAG researchers performed a genome-wide association study in a large pediatric group that identified a previously unknown association between T1D and a genetic variation on chromosome 16. This region contains KIAA0350, the gene product of which is predicted to be a sugar-binding protein. Subsequent follow-up studies (e.g. Concannon et al., 2008) have confirmed a link between this gene and type-1 diabetes, and our group is currently participating in a resequencing study of this region. This paper was published in Nature [hyperlink to ]
The Center uses microarrays to perform whole-genome analysis – microarrays are slides consisting of thousands to millions of tiny probes. They allow researchers to screen an individual’s genome for huge numbers of genetic markers called single nucleotide polymorphisms (SNPs). A SNP occurs when the DNA in two individuals in the population differs by a single nucleotide. For example, one individual may have an 'A' at a specific position, and another individual may have a 'C'. This can impact on the protein encoded by the DNA sequence (the gene) and increase gene risk.
An association typically means that a SNP is significantly more frequent in a patient-group than in controls. Once researchers know the location of a SNP that is more common in a group, they can examine the sequence of DNA from which it comes. In this way, they can check if it is part of a gene, or is close to a gene. The Center can simultaneously scan the genome for thousands of these associations at once – a powerful methodology called genome-wide association. At the Center, researchers have examined over 100,000 individuals.
Humans can also differ in the number of copies of each gene an individual has. These differences are called copy number variants. Rare alterations in a chromosome can lead to the gain or a loss of a copy. A duplication occurs when a fragment of DNA is gained – during copying, or when genes are shuffled at conception. The same process can cause a deletion, where a fragment of DNA is lost. Deletions and duplications of greater than 1,000 nucleotides are called copy number variants (CNVs).
The Center is equipped with the Illumina BeadArray System and utilizes both the Infinium and GoldenGate analytical methods. The Center's equipment includes multiple Tecan hardware systems and scanning instruments with integrative Laboratory Information Management System (LIMS). It uses several genotyping units from Affymetrix, adding flexibility to internal and collaborative projects to conduct studies on either platform.
- Elia J, Gai X, Xie HM, et al. (June 2010). "Rare structural variants found in attention-deficit hyperactivity disorder are preferentially associated with neurodevelopmental genes". Molecular Psychiatry 15 (6): 637–46. doi:10.1038/mp.2009.57. PMC 2877197. PMID 19546859.
- Fanta CH (March 2009). "Asthma". The New England Journal of Medicine 360 (10): 1002–14. doi:10.1056/NEJMra0804579. PMID 19264689.
- Sleiman PM, Flory J, Imielinski M, et al. (January 2010). "Variants of DENND1B associated with asthma in children". The New England Journal of Medicine 362 (1): 36–44. doi:10.1056/NEJMoa0901867. PMID 20032318.
- Wang K, Zhang H, Ma D, et al. (May 2009). "Common genetic variants on 5p14.1 associate with autism spectrum disorders". Nature 459 (7246): 528–33. doi:10.1038/nature07999. PMC 2943511. PMID 19404256.
- Glessner JT, Wang K, Cai G, et al. (May 2009). "Autism genome-wide copy number variation reveals ubiquitin and neuronal genes". Nature 459 (7246): 569–73. doi:10.1038/nature07953. PMC 2925224. PMID 19404257.
- Maris JM, Mosse YP, Bradfield JP, et al. (June 2008). "Chromosome 6p22 locus associated with clinically aggressive neuroblastoma". The New England Journal of Medicine 358 (24): 2585–93. doi:10.1056/NEJMoa0708698. PMC 2742373. PMID 18463370.
- Capasso M, Devoto M, Hou C, et al. (June 2009). "Common variations in BARD1 influence susceptibility to high-risk neuroblastoma". Nature Genetics 41 (6): 718–723. doi:10.1038/ng.374. PMC 2753610. PMID 19412175.
- Diskin SJ, Hou C, Glessner JT, et al. (June 2009). "Copy number variation at 1q21.1 associated with neuroblastoma". Nature 459 (7249): 987–91. doi:10.1038/nature08035. PMC 2755253. PMID 19536264.
- Kanetsky PA, Mitra N, Vardhanabhuti S, et al. (July 2009). "Common variation in KITLG and at 5q31.3 predisposes to testicular germ cell cancer". Nature Genetics 41 (7): 811–5. doi:10.1038/ng.393. PMC 2865677. PMID 19483682.
- Kugathasan S, Baldassano RN, Bradfield JP, et al. (October 2008). "Loci on 20q13 and 21q22 are associated with pediatric-onset inflammatory bowel disease". Nature Genetics 40 (10): 1211–5. doi:10.1038/ng.203. PMC 2770437. PMID 18758464.
- Wang K, Zhang H, Kugathasan S, et al. (March 2009). "Diverse genome-wide association studies associate the IL12/IL23 pathway with Crohn Disease". American Journal of Human Genetics 84 (3): 399–405. doi:10.1016/j.ajhg.2009.01.026. PMC 2668006. PMID 19249008.
- Imielinski M, Baldassano RN, Griffiths A, et al. (December 2009). "Common variants at five new loci associated with early-onset inflammatory bowel disease". Nature Genetics 41 (12): 1335–40. doi:10.1038/ng.489. PMC 3267927. PMID 19915574.
- Bhugra D (May 2005). "The global prevalence of schizophrenia". PLoS Medicine 2 (5): e151; quiz e175. doi:10.1371/journal.pmed.0020151. PMC 1140960. PMID 15916460.
- Goldner EM, Hsu L, Waraich P, Somers JM (November 2002). "Prevalence and incidence studies of schizophrenic disorders: a systematic review of the literature". Canadian Journal of Psychiatry 47 (9): 833–43. PMID 12500753.
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- Glessner JT, Reilly MP, Kim CE, et al. (June 2010). "Strong synaptic transmission impact by copy number variations in schizophrenia". Proceedings of the National Academy of Sciences of the United States of America 107 (23): 10584–9. doi:10.1073/pnas.1000274107. PMC 2890845. PMID 20489179.
- Grant SF, Hakonarson H, Schwartz S (April 2010). "Can the genetics of type 1 and type 2 diabetes shed light on the genetics of latent autoimmune diabetes in adults?". Endocrine Reviews 31 (2): 183–93. doi:10.1210/er.2009-0029. PMID 20007922.
- Concannon P, Onengut-Gumuscu S, Todd JA, et al. (October 2008). "A human type 1 diabetes susceptibility locus maps to chromosome 21q22.3". Diabetes 57 (10): 2858–61. doi:10.2337/db08-0753. PMC 2551699. PMID 18647951.
- "Single Nucleotide Polymorphisms". Dolan DNA Learning Center. Retrieved 2010-11-23.