Cfr10I/Bse634I

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Bse634I
PDB 1cfr EBI.jpg
crystal structure of citrobacter freundii restriction endonuclease cfr10i at 2.15 angstroms resolution.
Identifiers
Symbol Bse634I
Pfam PF07832
InterPro IPR012415

In molecular biology, the Cfr10I/Bse634I family of restriction endonucleases includes the type II restriction endonucleases Cfr10I and Bse634I. They exhibit a conserved tetrameric architecture that is of functional importance, wherein two dimers are arranged, back-to-back, with their putative DNA-binding clefts facing opposite directions. These clefts are formed between two monomers that interact, mainly via hydrophobic interactions supported by a few hydrogen bonds, to form a U-shaped dimer. Each monomer is folded to form a compact alpha-beta structure, whose core is made up of a five-stranded mixed beta-sheet. The monomer may be split into separate N-terminal and C-terminal subdomains at a hinge located in helix alpha3.[1] Both Cfr10I and Bse634I recognise the double-stranded sequence RCCGGY and cleave after the purine R.[2]

Recognition sequence     Cut
5' RCCGGY                5' ---R   CCGGY--- 3'
3' YGGCCR                3' ---YGGCC   R--- 5'

References[edit]

  1. ^ Grazulis S, Deibert M, Rimseliene R, Skirgaila R, Sasnauskas G, Lagunavicius A, Repin V, Urbanke C, Huber R, Siksnys V (February 2002). "Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence". Nucleic Acids Res. 30 (4): 876–85. doi:10.1093/nar/30.4.876. PMC 100338. PMID 11842098. 
  2. ^ Bozic D, Grazulis S, Siksnys V, Huber R (January 1996). "Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution". J. Mol. Biol. 255 (1): 176–86. doi:10.1006/jmbi.1996.0015. PMID 8568865. 

This article incorporates text from the public domain Pfam and InterPro IPR012415