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Cofactor may refer to any of the following:
- Cofactor in cryptography, the ratio between the order of a group and that of the subgroup.
- Cofactor (linear algebra), the signed minor of a matrix
- Minor (linear algebra), an alternative name for the determinant of a smaller matrix than that which it describes
- Cofactor (biochemistry), a substance that needs to be present in addition to an enzyme for a certain reaction to be catalysed
- Shannon cofactor, a term in Boole's (or Shannon's) expansion of a Boolean function
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