Cord blood-derived multipotent stem cell
Cord blood-derived multipotent stem cells (CB-SCs) are a type of multipotent stem cell from mammalian cord blood. They are identifiable by their ability to attach to the plastic surface of non-tissue cultured-treated Petri dishes.
Phenotypic characterization demonstrates that CB-SCs display embryonic cell markers (e.g., transcription factors OCT-4 and Nanog, stage-specific embryonic antigen (SSEA)-3, and SSEA-4) and leukocyte common antigen CD45, but they are negative for blood cell lineage markers (e.g., CD1a, CD3, CD4, CD8, CD11b, CD11c, CD13, CD14, CD19, CD20, CD34, CD41a, CD41b, CD83, CD90, CD105, and CD133).
Additionally, CB-SCs display very low immunogenicity as indicated by expression of a very low level of major histocompatibility complex (MHC) antigens and failure to stimulate the proliferation of allogeneic lymphocytes. They can give rise to three embryonic layer-derived cells in the presence of different inducers.
More specifically, CB-SCs tightly adhere to culture dishes with a large rounded morphology and are resistant to common detaching methods (trypsin/EDTA), making it easy to collect suspended lymphocytes after co-culture. CB-SCs are the active agent in stem cell educator therapy.
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