Cryo bio-crystallography

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Cryo bio-crystallography is the application of crystallography at cryogenic temperatures.

Basic principles[edit]

Cryo crystallography enables X-ray data collection at cryogenic, near liquid nitrogen temperatures (also called: N2).

  1. Crystals are transferred from the solution they have grown in (called mother liquor) to a hydrocarbon environment
  2. Crystals are mounted with a glass fiber (as opposed to a capillary)
  3. Crystals are cooled with a cold nitrogen stream on a diffraction apparatus to prevent the solvent freezing in the crystals thus maintaining crystallographic integrity.

Advantages[edit]

  1. Significant improvement of resolution in data collection
  2. Reduced or eliminated radiation damage in crystals

Usefulness and applications[edit]

Crystallography of large biological macromolecules can be achieved while maintaining their solution state. The most known example is the ribosome. [1]

References[edit]

  1. ^ Hope H (1988). "Cryocrystallography of biological macromolecules: a generally applicable method". Acta Crystallogr. B 44 (1): 22–26. doi:10.1107/s0108768187008632. PMID 3271102.