DAD1

Defender against cell death 1
Identifiers
Symbols	DAD1; OST2
External IDs	OMIM: 600243 MGI: 101912 HomoloGene: 1027 GeneCards: DAD1 Gene
EC number	2.4.1.119
Gene Ontology Molecular function • dolichyl-diphosphooligosaccharide-protein glycotransferase activity • transferase activity Cellular component • endoplasmic reticulum membrane • oligosaccharyltransferase complex • membrane • integral to membrane Biological process • blastocyst development • apoptosis • anti-apoptosis • response to nutrient • protein N-linked glycosylation via asparagine • protein N-linked glycosylation via asparagine • response to drug • post-translational protein modification • cellular protein metabolic process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1603	13135
Ensembl	ENSG00000129562	ENSMUSG00000022174
UniProt	P61803	P61804
RefSeq (mRNA)	NM_001344	XM_988624
RefSeq (protein)	NP_001335	XP_993718
Location (UCSC)	Chr 14: 23.03 – 23.06 Mb	Chr 14: 54.86 – 54.87 Mb
PubMed search	[1]	[2]

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 is an enzyme that in humans is encoded by the DAD1 gene.

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.

Interactions

DAD1 has been shown to interact with MCL1.

Further reading

• Yulug IG, See CG, Fisher EM, Ylug IG (1995). "The DAD1 protein, whose defect causes apoptotic cell death, maps to human chromosome 14.". Genomics 26 (2): 433–5. doi:10.1016/0888-7543(95)80241-D. PMID 7601483. 
• Apte SS, Mattei MG, Seldin MF, Olsen BR (1995). "The highly conserved defender against the death 1 (DAD1) gene maps to human chromosome 14q11-q12 and mouse chromosome 14 and has plant and nematode homologs.". FEBS Lett. 363 (3): 304–6. doi:10.1016/0014-5793(95)00321-Y. PMID 7737422. 
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. 
• Nakashima T, Sekiguchi T, Kuraoka A, et al. (1993). "Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.". Mol. Cell. Biol. 13 (10): 6367–74. PMC 364695. PMID 8413235. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=364695. 
• Kelleher DJ, Gilmore R (1997). "DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase.". Proc. Natl. Acad. Sci. U.S.A. 94 (10): 4994–9. doi:10.1073/pnas.94.10.4994. PMC 24619. PMID 9144178. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=24619. 
• Makishima T, Nakashima T, Nagata-Kuno K, et al. (1997). "The highly conserved DAD1 protein involved in apoptosis is required for N-linked glycosylation.". Genes Cells 2 (2): 129–41. doi:10.1046/j.1365-2443.1997.1070303.x. PMID 9167970. 
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
• Zhong XP, Krangel MS (1999). "Enhancer-blocking activity within the DNase I hypersensitive site 2 to 6 region between the TCR alpha and Dad1 genes.". J. Immunol. 163 (1): 295–300. PMID 10384128. 
• Makishima T, Yoshimi M, Komiyama S, et al. (2000). "A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family.". J. Biochem. 128 (3): 399–405. PMID 10965038. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801. 
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Shibatani T, David LL, McCormack AL, et al. (2005). "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits.". Biochemistry 44 (16): 5982–92. doi:10.1021/bi047328f. PMID 15835887.