DDB1

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Damage-specific DNA binding protein 1, 127kDa
Protein DDB1 PDB 2b5l.png
PDB rendering based on 2b5l.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols DDB1 ; DDBA; UV-DDB1; XAP1; XPCE; XPE; XPE-BF
External IDs OMIM600045 MGI1202384 HomoloGene1448 GeneCards: DDB1 Gene
Orthologs
Species Human Mouse
Entrez 1642 13194
Ensembl ENSG00000167986 ENSMUSG00000024740
UniProt Q16531 Q3U1J4
RefSeq (mRNA) NM_001923 NM_015735
RefSeq (protein) NP_001914 NP_056550
Location (UCSC) Chr 11:
61.07 – 61.11 Mb
Chr 19:
10.61 – 10.63 Mb
PubMed search [2] [3]

DNA damage-binding protein 1 is a protein that in humans is encoded by the DDB1 gene.[1][2][3]

Function[edit]

This gene encodes the large subunit of DNA damage-binding protein that is a heterodimer composed of a large and a small (DDB2) subunit. As its name suggests, DDB1 was initially implicated in the process of a specific type of DNA repair known as nucleotide excision repair. Since then, researchers have found that DDB1 primarily functions as a core component of the CUL4A- and CUL4B-based E3 ubiquitin ligase complexes. DDB1 serves as a bridge or adaptor protein which interacts with dozens of proteins known as DDB1 and CUL4-associated factors (DCAFs).[4] These DCAFs are often ubiquitin ligase substrates and regulate numerous essential processes in the cell including DNA repair (DDB2), DNA replication, chromatin remodeling (Cdt2) and more.

Interactions[edit]

DDB1 has been shown to interact with Transcription initiation protein SPT3 homolog,[5] GCN5L2,[6] DDB2,[7][8] CUL4A,[8] CUL4B[8] and P21.[9]

References[edit]

  1. ^ Dualan R, Brody T, Keeney S, Nichols AF, Admon A, Linn S (Feb 1996). "Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein". Genomics 29 (1): 62–9. doi:10.1006/geno.1995.1215. PMID 8530102. 
  2. ^ Seki N, Hayashi A, Hattori A, Kozuma S, Sasaki M, Suzuki Y, Sugano S, Muramatsu M, Saito T (Jan 2000). "cDNA cloning, tissue expression, and chromosomal assignment of a mouse gene, encoding a 127 kDa UV-damaged DNA binding protein which is defective in XPE cells". DNA Res 6 (5): 319–22. doi:10.1093/dnares/6.5.319. PMID 10574459. 
  3. ^ "Entrez Gene: DDB1 damage-specific DNA binding protein 1, 127kDa". 
  4. ^ [1]
  5. ^ Martinez E, Palhan VB, Tjernberg A, Lymar ES, Gamper AM, Kundu TK, Chait BT, Roeder RG (October 2001). "Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo". Mol. Cell. Biol. 21 (20): 6782–95. doi:10.1128/MCB.21.20.6782-6795.2001. PMC 99856. PMID 11564863. 
  6. ^ Huang J, Chen J (July 2008). "VprBP targets Merlin to the Roc1-Cul4A-DDB1 E3 ligase complex for degradation". Oncogene 27 (29): 4056–64. doi:10.1038/onc.2008.44. PMID 18332868. 
  7. ^ Bergametti F, Sitterlin D, Transy C (July 2002). "Turnover of hepatitis B virus X protein is regulated by damaged DNA-binding complex". J. Virol. 76 (13): 6495–501. doi:10.1128/JVI.76.13.6495-6501.2002. PMC 136256. PMID 12050362. 
  8. ^ a b c Guerrero-Santoro J, Kapetanaki MG, Hsieh CL, Gorbachinsky I, Levine AS, Rapić-Otrin V (July 2008). "The cullin 4B-based UV-damaged DNA-binding protein ligase binds to UV-damaged chromatin and ubiquitinates histone H2A". Cancer Res. 68 (13): 5014–22. doi:10.1158/0008-5472.CAN-07-6162. PMID 18593899. 
  9. ^ Abbas T, Sivaprasad U, Terai K, Amador V, Pagano M, Dutta A (September 2008). "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex". Genes Dev. 22 (18): 2496–506. doi:10.1101/gad.1676108. PMC 2546691. PMID 18794347. 

Further reading[edit]