DLG2

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Discs, large homolog 2 (Drosophila)
Protein DLG2 PDB 1iu0.png
PDB rendering based on 1iu0.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols DLG2 ; PPP1R58; PSD-93; PSD93; chapsyn-110
External IDs OMIM603583 MGI1344351 HomoloGene1046 GeneCards: DLG2 Gene
RNA expression pattern
PBB GE DLG2 206253 at tn.png
PBB GE DLG2 gnf1h02273 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 1740 23859
Ensembl ENSG00000150672 ENSMUSG00000052572
UniProt Q15700 Q91XM9
RefSeq (mRNA) NM_001142699 NM_001243046
RefSeq (protein) NP_001136171 NP_001229975
Location (UCSC) Chr 11:
83.17 – 85.34 Mb
Chr 7:
91.09 – 92.45 Mb
PubMed search [1] [2]

Disks large homolog 2 (DLG2) also known as channel-associated protein of synapse-110 (chapsyn-110) or postsynaptic density protein 93 (PSD-93) is a protein that in humans is encoded by the DLG2 gene.[1][2]

Function[edit]

Chapsyn-110/PSD-93 a member of the membrane-associated guanylate kinase (MAGUK) family. The protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Alternatively spliced transcript variants encoding distinct isoforms have been described but their full-length nature has yet to be completely determined.[3]

Model organisms[edit]

Model organisms have been used in the study of DLG2 function. A knockout mouse line, called Dlg2tm1Dsb was generated.[11][12] Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[9][13] Twenty four tests were carried out on homozygous mutant mice and five significant abnormalities were observed.[9] Both sexes had atypical indirect calorimetry and DEXA parameters. Females also had decreased body weight, decreased circulating HDL cholesterol levels, and increased susceptibility to bacterial infection.[9]

Interactions[edit]

DLG2 has been shown to interact with GRIN2B,[14][15] KCNJ12.[16]

References[edit]

  1. ^ Kim E, Cho KO, Rothschild A, Sheng M (December 1996). "Heteromultimerization and NMDA receptor-clustering activity of Chapsyn-110, a member of the PSD-95 family of proteins". Neuron 17 (1): 103–13. doi:10.1016/S0896-6273(00)80284-6. PMID 8755482. 
  2. ^ Stathakis DG, Lee D, Bryant PJ (January 1999). "Fine-scale physical map of the 11q21 region surrounding the human DLG2 locus, the gene encoding Chapsyn-110". Genomics 54 (1): 186–8. doi:10.1006/geno.1998.5527. PMID 9806853. 
  3. ^ "Entrez Gene: DLG2 discs, large homolog 2, chapsyn-110 (Drosophila)". 
  4. ^ "Body weight data for Dlg2". Wellcome Trust Sanger Institute. 
  5. ^ "Indirect calorimetry data for Dlg2". Wellcome Trust Sanger Institute. 
  6. ^ "DEXA data for Dlg2". Wellcome Trust Sanger Institute. 
  7. ^ "Clinical chemistry data for Dlg2". Wellcome Trust Sanger Institute. 
  8. ^ "Citrobacter infection data for Dlg2". Wellcome Trust Sanger Institute. 
  9. ^ a b c d Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. 
  10. ^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
  11. ^ McGee AW, Topinka JR, Hashimoto K, Petralia RS, Kakizawa S, Kauer FW, Aguilera-Moreno A, Wenthold RJ, Kano M, Bredt DS, Kauer F (2001). "PSD-93 knock-out mice reveal that neuronal MAGUKs are not required for development or function of parallel fiber synapses in cerebellum". J. Neurosci. 21 (9): 3085–91. PMID 11312293. 
  12. ^ "Mouse Genome Informatics". 
  13. ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353. 
  14. ^ Inanobe A, Fujita A, Ito M, Tomoike H, Inageda K, Kurachi Y (June 2002). "Inward rectifier K+ channel Kir2.3 is localized at the postsynaptic membrane of excitatory synapses". Am. J. Physiol., Cell Physiol. 282 (6): C1396–403. doi:10.1152/ajpcell.00615.2001. PMID 11997254. 
  15. ^ Irie M, Hata Y, Takeuchi M, Ichtchenko K, Toyoda A, Hirao K, Takai Y, Rosahl TW, Südhof TC (September 1997). "Binding of neuroligins to PSD-95". Science 277 (5331): 1511–5. doi:10.1126/science.277.5331.1511. PMID 9278515. 
  16. ^ Leonoudakis D, Conti LR, Anderson S, Radeke CM, McGuire LM, Adams ME, Froehner SC, Yates JR, Vandenberg CA (May 2004). "Protein trafficking and anchoring complexes revealed by proteomic analysis of inward rectifier potassium channel (Kir2.x)-associated proteins". J. Biol. Chem. 279 (21): 22331–46. doi:10.1074/jbc.M400285200. PMID 15024025. 

Further reading[edit]

  • Kim E, Niethammer M, Rothschild A, et al. (1995). "Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases.". Nature 378 (6552): 85–8. doi:10.1038/378085a0. PMID 7477295. 
  • Brenman JE, Chao DS, Gee SH, et al. (1996). "Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha1-syntrophin mediated by PDZ domains.". Cell 84 (5): 757–67. doi:10.1016/S0092-8674(00)81053-3. PMID 8625413. 
  • Hsueh YP, Kim E, Sheng M (1997). "Disulfide-linked head-to-head multimerization in the mechanism of ion channel clustering by PSD-95.". Neuron 18 (5): 803–14. doi:10.1016/S0896-6273(00)80319-0. PMID 9182804. 
  • Niethammer M, Valtschanoff JG, Kapoor TM, et al. (1998). "CRIPT, a novel postsynaptic protein that binds to the third PDZ domain of PSD-95/SAP90.". Neuron 20 (4): 693–707. doi:10.1016/S0896-6273(00)81009-0. PMID 9581762. 
  • Leonard AS, Davare MA, Horne MC, et al. (1998). "SAP97 is associated with the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor GluR1 subunit.". J. Biol. Chem. 273 (31): 19518–24. doi:10.1074/jbc.273.31.19518. PMID 9677374. 
  • Butz S, Okamoto M, Südhof TC (1998). "A tripartite protein complex with the potential to couple synaptic vesicle exocytosis to cell adhesion in brain.". Cell 94 (6): 773–82. doi:10.1016/S0092-8674(00)81736-5. PMID 9753324. 
  • Brenman JE, Topinka JR, Cooper EC, et al. (1998). "Localization of postsynaptic density-93 to dendritic microtubules and interaction with microtubule-associated protein 1A.". J. Neurosci. 18 (21): 8805–13. PMID 9786987. 
  • Craven SE, El-Husseini AE, Bredt DS (1999). "Synaptic targeting of the postsynaptic density protein PSD-95 mediated by lipid and protein motifs.". Neuron 22 (3): 497–509. doi:10.1016/S0896-6273(00)80705-9. PMID 10197530. 
  • Sans N, Petralia RS, Wang YX, et al. (2000). "A developmental change in NMDA receptor-associated proteins at hippocampal synapses.". J. Neurosci. 20 (3): 1260–71. PMID 10648730. 
  • Garcia RA, Vasudevan K, Buonanno A (2000). "The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses.". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3596–601. doi:10.1073/pnas.070042497. PMC 16285. PMID 10725395. 
  • Husi H, Ward MA, Choudhary JS, et al. (2000). "Proteomic analysis of NMDA receptor-adhesion protein signaling complexes.". Nat. Neurosci. 3 (7): 661–9. doi:10.1038/76615. PMID 10862698. 
  • Firestein BL, Craven SE, Bredt DS (2001). "Postsynaptic targeting of MAGUKs mediated by distinct N-terminal domains.". NeuroReport 11 (16): 3479–84. doi:10.1097/00001756-200011090-00016. PMID 11095503. 
  • Haraguchi K, Satoh K, Yanai H, et al. (2001). "The hDLG-associated protein DAP interacts with dynein light chain and neuronal nitric oxide synthase.". Genes Cells 5 (11): 905–911. doi:10.1046/j.1365-2443.2000.00374.x. PMID 11122378. 
  • DeMarco SJ, Strehler EE (2001). "Plasma membrane Ca2+-atpase isoforms 2b and 4b interact promiscuously and selectively with members of the membrane-associated guanylate kinase family of PDZ (PSD95/Dlg/ZO-1) domain-containing proteins.". J. Biol. Chem. 276 (24): 21594–600. doi:10.1074/jbc.M101448200. PMID 11274188. 
  • Russwurm M, Wittau N, Koesling D (2002). "Guanylyl cyclase/PSD-95 interaction: targeting of the nitric oxide-sensitive alpha2beta1 guanylyl cyclase to synaptic membranes.". J. Biol. Chem. 276 (48): 44647–52. doi:10.1074/jbc.M105587200. PMID 11572861. 
  • Weitzdoerfer R, Dierssen M, Fountoulakis M, Lubec G (2002). "Fetal life in Down syndrome starts with normal neuronal density but impaired dendritic spines and synaptosomal structure.". J. Neural Transm. Suppl. (61): 59–70. PMID 11771761.