Dafnousia (Greek: Δαφνούσια) is a former municipality in Phthiotis, Greece. Since the 2011 local government reform it is part of the municipality Lokroi, of which it is a municipal unit. In 2011 its population was 3,629. The seat of the municipality was in Livanates where three quarters of the population live. Dafnousia borders on the municipal unit of Atalanti to the south, and Agios Konstantinos to the west.