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Decellularization is a processed tissue treatment by which cells are discharged. It is a process often used in the creation of bioartificial organs.

The consequential cellularly neutralized parenchyma implies several advantages to effectively restoring an unhealthy, missing, or damaged membrane. For example, donated tissue frequently contributes to immunological rejection. Applying this technique renders the transplant successful.


In brief, the first step involves the application of a specialized detergent known to be an efficient solubilizer, without affecting the integrity of the protein in the tissue. Then, a recombinant endonuclease is used to degrade nucleic acids .

The second step relates to ensuring decellularization is complete.

Step three is meant to preserve, protect, and hold the tissue in conditions similar to body conditions, ready to be utilized.

Perfusion Decellularization[edit]

Perfusion decellularization implies a process where the detergent is continuously pumped through the target organ, continuously removing living cells until the remaining matrix remains.[1]

Immersion Decellularization[edit]

Immersion decellularization refers to the process where the organ is submerged in a solution that kills the living cells, leaving the protein matrix intact.

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