Demineralizing (silk worm cocoon)
Demineralizing has the potential to be used in the silk sector enabling wet reeling of Wild Silk moth cocoons by removing the mineral layer present in these cocoons. This technique is not like degumming where the gum of the fibroin fibres is removed what would lead to a tangled cocoon. With "demineralizing" the gum and structure of the cocoon is kept intact enabling the cocoons to be wet reeled. This could allow a new silk industry in areas which have not the conditions or infrastructure for raising the domesticated silk worm Bombyx mori, possibly generating a revolutionary new income stream and alleviating poverty
Standard demineralizing solution
An aqueous 1 M solution of ethylenediaminetetraacetic acid (EDTA) is used adjusted to pH 10 with saturated sodium hydroxide solution. The cocoons are immersed in this solution for 72 h at 40°C and gently stirred. During the process, the cocoons are kept under the surface of the solution by means of a plastic mesh, using a partial vacuum to encourage penetration of the solution. Afterwards, the cocoons are thoroughly washed with running tap water (three times 3 h) and subsequently reeled in tap water at room temperature using the standard method for Bombyx mori. The method described here forms the basis of a patent application 
Other solutions useful for demineralizing
Citric acid (present in lemon juice, orange juice, pineapple juice), formic acid, aluminum nitrate,and phosphate ions are all known to remove calcium oxalate by chelating calcium ions or by directly dissolving it.
- PhysOrg (17 May 2011). "Reeling in a wild silk harvest". PhysOrg. Retrieved 26 May 2011.
- Science Daily (22 May 2011). "New Method of Unreeling Cocoons Could Extend Silk Industry Beyond Asia". ScienceDaily. Retrieved 26 May 2011.
- Sindya N. Bhanoo (20 May 2011). "Silk Production Takes a Walk on the Wild Side". New York Times. Retrieved 26 May 2011.
- Gheysens, T; Collins, A; Raina, S; Vollrath, F; Knight, D (2011). "Demineralization enables reeling of Wild Silkmoth cocoons". Biomacromolecules (American Chemical Society) 12 (6): 2257–66. doi:10.1021/bm2003362. PMID 21491856. Retrieved 26 May 2011.
- GB 1018269.9, Gheysens, T, "Demineralization Enables Reeling of Wild Silkmoth Cocoons" (patent pending)