Developmental Studies Hybridoma Bank

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Developmental Studies Hybridoma Bank (DSHB)
DSHB logo 2015.png
Founded 1986
Type Non-profit organization, Biological resource center
Location
Services Bank and distribute hybridomas and cell products

The Developmental Studies Hybridoma Bank (DSHB) is a non-profit, global hybridoma bank. The DSHB is a National Resource established by the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) to bank and distribute at cost hybridomas and cell products to the general scientific community.

Mission[edit]

The mission of the DSHB is four-fold:

  • Keep product prices low to facilitate research (Currently 35.00 USD per ml of supernatant)
  • Serve as a repository to relieve scientist of the time and expense of distributing hybridomas and the monoclonal antibodies (MAbs) they produce
  • Assure the scientific community that MAbs with limited demand remain available
  • Maintain the highest product quality and provide prompt customer service and knowledgeable technical assistance.

Description[edit]

The DSHB is directed by David R. Soll at the University of Iowa. There are currently 2500 hybridomas in the DSHB collection.[1] They have obtained hybridoma collections from a variety of individuals and institutions including the Muscular Dystrophy Association, the National Cancer Institute, the NIH Common Fund, and the European Molecular Biology Laboratory (EMBL) and they eagerly await new collections. The DSHB has begun developing complex monoclonal antibody microarrays for specific targets. First time customers must agree to the DSHB terms of usage that products will be used for research purposes only, and that they cannot be commercialized or distributed to a third party. Researchers also agree to acknowledge both the DSHB and the contributing investigator in publications that benefit from the use of DSHB products and provide the DSHB copies or citations of all publications. It is free for individuals or institutions to deposit hybridomas for distribution and archiving, there is no maximum or minimum deposit. As the depositor, you have the option to allow the DSHB to distribute the hyrbidoma cells, or just the cell products. As a non-profit, the organization covers the operating costs of maintaining, improving and producing products in the collection by selling the products at cost.

History[edit]

The DSHB was created in 1986 by the Institute of Child Health and Human Development (NIH) to bank and distribute hybridomas and the monoclonal antibodies (MAbs) they produce to the general scientific community in order to facilitate research. The DSHB has been self-funded since 1996. One of the first hybridomas deposited with the DSHB was MF 20 which recognizes all isoforms of skeletal myosin heavy chain. In April 1986, Dr. Donald Fischman, Cornell Medical School, deposited MF 20 with the DSHB. Nearly 30 years later, MF 20 remains one of the top ten most requested monoclonal antibodies.

Popular Collections[edit]

Popular targets that the DSHB provides products for include:

  • Drosophila antigens
  • Cell markers
  • Dictyostelium antigens
  • Cytoskeletal elements
  • Transcription factors
  • Cluster Determinant (CD) antigens
  • Muscular Dystrophy-associated proteins
  • NCI – Cancer targets
  • Neurodevelopment markers
  • Cardiac development proteins
  • Extracellular matrix proteins
  • Cell adhesion receptors
  • Cell signaling
  • Epitope tags
  • Stem cells
  • Skeletal muscle
  • Enzymes
  • Human
  • C. elegans
  • Xenopus
  • Nucleus
  • Microbes (viruses, bacteria, fungi)

Noteworthy Depositors[edit]

Nobel Prize and Alfred P. Sloan Prize winner Michael J. Bishop deposited the anti c-MYC hybridoma 9e10 [2]

Nobel Prize winner Sir John Gurdon deposited MyoD clone D7F2[3]

Nobel Prize winner Eric F. Wieschaus deposited 7 hybridomas[4]

National Academy of Sciences Members who have deposited hybridomas


The National Cancer Institute deposited 463 Monoclonal antibodies from the Protein Capture Reagents Program.

References[edit]

External links[edit]