Jones-Konihowski was born in Vancouver, British Columbia, and raised in Saskatoon, Saskatchewan. She graduated from the College of Education at the University of Saskatchewan. Diane took her first international medal at the 1969 Pacific Conference Games, a bronze in the high jump. She competed for Canada at the 1972 Summer Olympics, placing tenth and the 1976 Summer Olympics, placing sixth. Considered to be a medal contender for the 1980 Summer Olympics in Moscow, a third appearance at the Olympics failed to materialize as Canada was one of the countries that chose to boycott the games due to the Soviet intervention in Afghanistan. Although Jones-Konihowski considered competing as an individual, she ultimately decided against it. She won a gold medal in the pentathlon at the 1975 and 1979 Pan Am Games and 1978 Commonwealth Games. She was also the winner of the pentathlon at the Liberty Bell Classic (alternate Olympic competition) in 1980. Two weeks after the Moscow Olympics, Diane competed in the pentathlon in Germany; she won the gold beating all the Olympic Medallist.  She retired from competition in 1983. She was Chef de Mission of the 2000 Canadian Olympic Team in Sydney, Australia.