Dichlorofluorescein

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Dichlorofluorescein
Dichlorofluorescein.svg
Identifiers
CAS number 76-54-0 YesY
PubChem 64944
ChemSpider 58471 YesY
ChEBI CHEBI:51596 YesY
ChEMBL CHEMBL1908059
Jmol-3D images Image 1
Properties
Molecular formula C20H10Cl2O5
Molar mass 401.20 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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Infobox references

Dichlorofluorescein (DCF) is an organic dye of the fluorescein family, being substituted at the 2 and 7 positions by chloride.

It is used as an indicator for argentometry by Fajans method.[1][2]

It is also used in the cellular antioxidant activity (CAA) assay. Dichlorofluorescin is a probe that is trapped within cells and is easily oxidized to fluorescent dichlorofluorescein. The method measures the ability of compounds to prevent the formation of DCF by 2,2'-Azobis(2-amidinopropane) dihydrochloride (ABAP)-generated peroxyl radicals in human hepatocarcinoma HepG2 cells.[3]

References[edit]

  1. ^ Kolthoff, I. M.; Lauer, W. M.; Sunde, C. J. (1929). "The Use of Dichlorofluorescein as an Adsorption Indicator for the Argentometric Titration of Chlorides". Journal of the American Chemical Society 51 (11): 3273. doi:10.1021/ja01386a014. 
  2. ^ Bambach, Karl; Rider, T. H. (1935). "Volumetric Determinations of Halides: Use of Dichlorofluorescein as an Adsorption Indicator". Industrial & Engineering Chemistry Analytical Edition 7 (3): 165. doi:10.1021/ac50095a012. 
  3. ^ Wolfe, K. L.; Liu, R. H. (2007). "Cellular Antioxidant Activity (CAA) Assay for Assessing Antioxidants, Foods, and Dietary Supplements". Journal of Agricultural and Food Chemistry 55 (22): 8896–8907. doi:10.1021/jf0715166. PMID 17902627.  edit