The rail link was completed in April 2005. In preparation for the opening of Hong Kong Disneyland theme park on 12 September, the line started operating on 1 August 2005. The rolling stock is distinctive (see also below) on account of its Mickey Mouse windows, interior couch seating, and Disneyland figurines displayed in the carriages.
On 4 September 2005, Disneyland Resort Line served nearly 40,000 passengers and peak patronage occurs around 0800-1000 and 2100–2300 hours.
The Disneyland Resort Line is 3.5 kilometres long, and has a travel time of three and a half minutes. The MTR designed a new driverless train and produced it by refitting existing M-Trains for the line which is designed with a Disney theme in mind. Bronze statues of well-known Disney characters are included inside of the trains, such as Mickey Mouse and Donald Duck, and the windows are shaped not like squares but like Mickey Mouse's head.
Some of the M-Train cars used in the Disneyland Resort Line were originally ordered from 1994-1998 as subtype H-Stock train (Phase 3 EMU, A/C 270-291, B/C 486-496). MTR contracted Alstom for these trains, but they were made in England (as compared to the Phase 1 and 2 trains ordered from 1979–1989 and made by Metro-Cammell (also in England) which was acquired by GEC Alsthom (now Alstom) in 1989). Units A/C274 A/C281 A/C284 A/C289 A/C291 and B/C490 are now used on the Disneyland Resort Line.