Dispersin B
| Dispersin B | |
|---|---|
 Ribbon diagram of Dispersin B |
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| Identifiers | |
| Symbol | DspB |
| PDB | 1YHT |
| RefSeq | YP_001449721 |
| UniProt | Q840G9 |
| Other data | |
| EC number | 3.2.1.52 |
Dispersin B (EC 3.2.1.52) (also known as DspB or DispersinB) is a 42 kDa glycoside hydrolase enzyme produce by the periodontal pathogen Aggregatibacter actinomycetemcomitans.[1] Dispersin B catalyzes the hydrolysis of poly-N-acetylglucosamine, a sticky extracellular polysaccharide produce by various Gram-positive bacteria including Staphylococcus aureus and Staphylococcus epidermidis, as well as many Gram-negative bacteria including Escherichia coli, Yersinia pestis and Bordetella spp. Poly-N-acetylglucosamine is sometimes called polysaccharide intercellular adhesin or PIA.
Contents |
Function [edit]
Dispersin B plays a role in the detachment and dispersal of bacterial cells from Aggregatibacter actinomycetemcomitans biofilms.[1]
Structure [edit]
The three dimensional structure of dispersin B has been determined.[2]
Applications [edit]
Dispersin B may be useful for treating and preventing biofilm-associated infections caused by poly-N-acetylglucosamine-producing bacteria.
Commercial development [edit]
Dispersin B is being commercially developed as a wound care gel and medical device coating by Kane Biotech, Inc., a Canadian biotech company. Pharmaceutical-grade dispersin B has been successfully manufactured by BioVectra, Inc., Charlottetown, P.E.I., Canada, and is currently undergoing biocompatibility testing. Clinical trials to test the effectiveness of dispersin B for the treatment and prevention of diabetic foot ulcers and pressure sores should begin in 2010.
References [edit]
- ^ a b Kaplan JB, Ragunath C, Ramasubbu N, Fine DH (August 2003). "Detachment of Actinobacillus actinomycetemcomitans biofilm cells by an endogenous beta-hexosaminidase activity". J. Bacteriol. 185 (16): 4693–8. doi:10.1128/JB.185.16.4693-4698.2003. PMC 166467. PMID 12896987.
- ^ PDB 1YHT; Ramasubbu N, Thomas LM, Ragunath C, Kaplan JB (June 2005). "Structural analysis of dispersin B, a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans". J. Mol. Biol. 349 (3): 475–86. doi:10.1016/j.jmb.2005.03.082. PMID 15878175.
