Ribbon diagram of Dispersin B
|PDB||1YHT (RCSB PDB PDBe PDBj)|
Dispersin B (EC 220.127.116.11) (also known as DspB or DispersinB) is a 42 kDa glycoside hydrolase enzyme produce by the periodontal pathogen Aggregatibacter actinomycetemcomitans. Dispersin B catalyzes the hydrolysis of poly-N-acetylglucosamine, a sticky extracellular polysaccharide produce by various Gram-positive bacteria including Staphylococcus aureus and Staphylococcus epidermidis, as well as many Gram-negative bacteria including Escherichia coli, Yersinia pestis and Bordetella spp. Poly-N-acetylglucosamine is sometimes called polysaccharide intercellular adhesin or PIA.
The three dimensional structure of dispersin B has been determined.
Dispersin B may be useful for treating and preventing biofilm-associated infections caused by poly-N-acetylglucosamine-producing bacteria.
Dispersin B is being commercially developed as a wound care gel and medical device coating by Kane Biotech, Inc., a Canadian biotech company. Pharmaceutical-grade dispersin B has been successfully manufactured by BioVectra, Inc., Charlottetown, P.E.I., Canada, and is currently undergoing biocompatibility testing. Clinical trials to test the effectiveness of dispersin B for the treatment and prevention of diabetic foot ulcers and pressure sores should begin in 2010.
- Kaplan JB, Ragunath C, Ramasubbu N, Fine DH (August 2003). "Detachment of Actinobacillus actinomycetemcomitans biofilm cells by an endogenous beta-hexosaminidase activity". J. Bacteriol. 185 (16): 4693–8. doi:10.1128/JB.185.16.4693-4698.2003. PMC 166467. PMID 12896987.
- PDB 1YHT; Ramasubbu N, Thomas LM, Ragunath C, Kaplan JB (June 2005). "Structural analysis of dispersin B, a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans". J. Mol. Biol. 349 (3): 475–86. doi:10.1016/j.jmb.2005.03.082. PMID 15878175.