EIF1AX

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Eukaryotic translation initiation factor 1A, X-linked
Protein EIF1AX PDB 1d7q.png
PDB rendering based on 1d7q.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols EIF1AX ; EIF1A; EIF1AP1; EIF4C; eIF-1A; eIF-4C
External IDs OMIM300186 MGI1913485 HomoloGene20364 GeneCards: EIF1AX Gene
RNA expression pattern
PBB GE EIF1AX 201016 at tn.png
PBB GE EIF1AX 201017 at tn.png
PBB GE EIF1AX 201019 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 1964 66235
Ensembl ENSG00000173674 ENSMUSG00000067194
UniProt P47813 Q8BMJ3
RefSeq (mRNA) NM_001412 NM_025437
RefSeq (protein) NP_001403 NP_079713
Location (UCSC) Chr X:
20.14 – 20.16 Mb
Chr X:
159.37 – 159.39 Mb
PubMed search [1] [2]

Eukaryotic translation initiation factor 1A, X-chromosomal is a protein that in humans is encoded by the EIF1AX gene.[1][2][3]

Mammalian 17-kDa eukaryotic initiation factor, eIF1A (formerly designated eIF-4C), is essential for transfer of the initiator Met-tRNAf (as Met-tRNAf·eIF2·GTP ternary complex) to 40 S ribosomal subunits in the absence of mRNA to form the 40 S preinitiation complex (40 S·Met-tRNAf·eIF2·GTP). Furthermore, eIF1A acts catalytically in this reaction to mediate highly efficient transfer of the Met-tRNAf·eIF2·GTP ternary complex to 40 S ribosomal subunits. The 40 S complex formed is free of eIF1A which indicates that its role in 40 S preinitiation complex formation is not to stabilize the binding of Met-tRNAf to 40 S ribosomes. Additionally, the eIF1A-mediated 40 S initiation complex formed in the presence of AUG codon efficiently joins 60 S ribosomal subunits in an eIF5-dependent reaction to form a functional 80 S initiation complex. Though found in some reports, eIF1A probably plays no role either in the subunit joining reaction or in the generation of ribosomal subunits from 80 S ribosomes. The major function of eIF1A is to mediate the transfer of Met-tRNAf to 40 S ribosomal subunits to form the 40 S preinitiation complex.[4]

This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.[3]

Mutations in this gene have been recurrently seen associated to cases of uveal melanoma with disomy 3 (http://www.ncbi.nlm.nih.gov/pubmed/23793026).

Interactions[edit]

EIF1AX has been shown to interact with IPO13.[5]

References[edit]

  1. ^ Dever TE, Wei CL, Benkowski LA, Browning K, Merrick WC, Hershey JW (Mar 1994). "Determination of the amino acid sequence of rabbit, human, and wheat germ protein synthesis factor eIF-4C by cloning and chemical sequencing". J Biol Chem 269 (5): 3212–8. PMID 8106356. 
  2. ^ Lahn BT, Page DC (Nov 1997). "Functional coherence of the human Y chromosome". Science 278 (5338): 675–80. doi:10.1126/science.278.5338.675. PMID 9381176. 
  3. ^ a b "Entrez Gene: EIF1AX eukaryotic translation initiation factor 1A, X-linked". 
  4. ^ Umadas Maitra, Jayanta Chaudhuri; Jayanta Chaudhuri; Kausik Si (21 March 1997). "Function of Eukaryotic Translation Initiation Factor 1A (eIF1A) (Formerly Called eIF-4C) in Initiation of Protein Synthesis". The Journal of BIological Chemistry 272 (12): 7883–7891. doi:10.1074/jbc.272.12.7883. Retrieved 19 February 2012. 
  5. ^ Mingot, J M; Kostka S; Kraft R; Hartmann E; Görlich D (Jul 2001). "Importin 13: a novel mediator of nuclear import and export". EMBO J. (England) 20 (14): 3685–94. doi:10.1093/emboj/20.14.3685. ISSN 0261-4189. PMC 125545. PMID 11447110. 

Further reading[edit]