Eukaryotic translation initiation factor 4 gamma 2 (also called p97, NAT1, and DAP-5) is a protein that in humans is encoded by the EIF4G2gene.
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
^Yamanaka S, Poksay KS, Arnold KS, Innerarity TL (March 1997). "A novel translational repressor mRNA is edited extensively in livers containing tumors caused by the transgene expression of the apoB mRNA-editing enzyme". Genes Dev11 (3): 321–33. doi:10.1101/gad.11.3.321. PMID9030685.
Shaughnessy JD, Jenkins NA, Copeland NG (1997). "cDNA cloning, expression analysis, and chromosomal localization of a gene with high homology to wheat eIF-(iso)4F and mammalian eIF-4G.". Genomics39 (2): 192–7. doi:10.1006/geno.1996.4502. PMID9027506.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK (1999). "Cap-dependent translation initiation in eukaryotes is regulated by a molecular mimic of eIF4G.". Mol. Cell3 (6): 707–16. doi:10.1016/S1097-2765(01)80003-4. PMID10394359.
Marcotrigiano J, Lomakin IB, Sonenberg N, et al. (2001). "A conserved HEAT domain within eIF4G directs assembly of the translation initiation machinery.". Mol. Cell7 (1): 193–203. doi:10.1016/S1097-2765(01)00167-8. PMID11172724.
Marissen WE, Gradi A, Sonenberg N, Lloyd RE (2001). "Cleavage of eukaryotic translation initiation factor 4GII correlates with translation inhibition during apoptosis.". Cell Death Differ.7 (12): 1234–43. doi:10.1038/sj.cdd.4400750. PMID11175261.
Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.". Nat. Biotechnol.21 (5): 566–9. doi:10.1038/nbt810. PMID12665801.
Li Z, Hu CY, Mo BQ, et al. (2003). "[Effect of beta-carotene on gene expression of breast cancer cells]". Ai Zheng22 (4): 380–4. PMID12703993.
Qin H, Raught B, Sonenberg N, et al. (2004). "Phosphorylation screening identifies translational initiation factor 4GII as an intracellular target of Ca(2+)/calmodulin-dependent protein kinase I.". J. Biol. Chem.278 (49): 48570–9. doi:10.1074/jbc.M308781200. PMID14507913.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet.36 (1): 40–5. doi:10.1038/ng1285. PMID14702039.