Passenger rail services from Echuca to Balranald were withdrawn on 7 November 1975, with the last train a 153hp Walker railmotor. The Echuca to Kyabram service was withdrawn on 18 December 1975. The last train was also 153 hp Walker railmotor, the service being provided for school children and paid for by the Education Department. The Bendigo – Echuca – Deniliquin passenger service was withdrawn on 11 January 1979, with the last train being operated by a DERM. Toolamba – Echuca passenger services were withdrawn on 2 March 1981, leaving the town without any rail services.
A twice-weekly passenger service from Bendigo was reinstated in 1996. The passenger service was replaced with buses on 19 December 2004, pending an upgrade due to poor track condition, reopening in late 2006.
Passenger services on the line are operated by V/Line as extensions of services to Bendigo from Southern Cross station in Melbourne. Bold stations are termini, where some train services terminate; italic stations are always serviced; and stations with an asterisk (*) are staffed part-time.