20 May 1860|
Munich, German Confederation
|Died||13 August 1917
Munich, German Empire
|Alma mater||University of Munich|
|Doctoral advisor||Otto Fischer,
Adolf von Baeyer
|Known for||Mannich reaction|
|Notable awards||Nobel Prize in Chemistry (1907)|
Buchner was born in Munich to a physician and Doctor Extraordinary of Forensic Medicine. His older brother was Hans Ernst August Buchner. In 1884, he began studies of chemistry with Adolf von Baeyer and of botany with Professor C. von Naegeli, at the Botanic Institute in Munich. After a period working with Hermann Emil Fischer in Erlangen, Buchner was awarded a doctorate from the University of Munich in 1888.
The experiment for which Buchner won the Nobel Prize consisted of producing a cell-free extract of yeast cells and showing that this "press juice" could ferment sugar. This dealt yet another blow to vitalism by showing that the presence of living yeast cells was not needed for fermentation. The cell-free extract was produced by combining dry yeast cells, quartz and kieselguhr and then pulverizing the yeast cells with a pestle and mortar. This mixture would then become moist as the yeast cells' contents would come out of the cells. Once this step was done, the moist mixture would be put through a press and the resulting "press juice" had glucose, fructose, or maltose added and carbon dioxide was seen to evolve, sometimes for days. Microscopic investigation revealed no living yeast cells in the extract. Buchner hypothesized that yeast cells secrete proteins into their environment in order to ferment sugars, but it was later found that fermentation occurs inside the yeast cells. Maria Manasseina claimed to have discovered free-cell fermentation a generation earlier than Buchner.
Buchner received the Nobel Prize in Chemistry in 1907.
Buchner married Lotte Stahl in 1900. During World War I, Buchner served as a Major in a front-line field hospital at Focşani, Romania. He was wounded on August 3, 1917 and died of these wounds nine days later in Munich at age 57.
- Eduard Buchner (1897). "Alkoholische Gärung ohne Hefezellen (Vorläufige Mitteilung)". Berichte der Deutschen Chemischen Gesellschaft 30: 117–124. doi:10.1002/cber.18970300121.
- Eduard Buchner, Rudolf Rapp (1899). "Alkoholische Gärung ohne Hefezellen". Berichte der Deutschen Chemischen Gesellschaft 32 (2): 2086. doi:10.1002/cber.189903202123.
- Robert Kohler (1971). "The background to Eduard Buchner's discovery of cell-free fermentation". Journal of the History of Biology 4 (1): 35–61. doi:10.1007/BF00356976. PMID 11609437.
- Robert Kohler (1972). "The reception of Eduard Buchner's discovery of cell-free fermentation". Journal of the History of Biology 5 (2): 327–353. doi:10.1007/BF00346663. PMID 11610124.
- Asimov, Asimov's Biographical Encyclopedia of Science and Technology 2nd Revised edition
- Athel Cornish-Bowden (1999). "The Origins of Enzymology." // The Biochemist 19(2), 36–38.
- Jensen, William (2006). "The Origins of the Hirsch and Büchner Vacuum Filtration Funnels". Journal of Chemical Education 83 (9): 1283. Bibcode:2006JChEd..83.1283J. doi:10.1021/ed083p1283.[dead link]
- Ukrow, Rolf (2004). Nobelpreisträger Eduard Buchner (1860 – 1917) Ein Leben für die Chemie der Gärungen und - fast vergessen - für die organische Chemie (German). Berlin.
- Buchner's Nobel Lecture Cell Free Fermentation
- Buchner, Eduard (1897). "Alcoholic Fermentation Without Yeast Cells". Ber. Dt. Chem. Ges. 30: 117–124. (English translation of Buchner's "Alkoholische Gährung ohne Hefezellen")