Elizabethkingia meningoseptica

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Elizabethkingia meningoseptica
Elizabethkingia meningoseptica Blood agar plate.JPG
Wet raised colonies with clear margin and characteristic smell on Blood agar growth. Sometimes may not show yellow color. Vancomycin sensitivity (clearing around disk) and colistin resistance may lead to mistaking this organism as gram positive.
Scientific classification
Superdomain: Phylogenetica
Kingdom: Bacteria
Phylum: Bacteroidetes
Class: Flavobacteria
Order: Flavobacteriales
Family: Flavobacteriaceae
Genus: Elizabethkingia
Species: E. meningoseptica
Binomial name
Elizabethkingia meningoseptica
(King, 1959) Kim et al., 2005
Synonyms
  • Flavobacterium meningosepticum King 1959
  • Chryseobacterium meningosepticum (King 1959) Vandamme et al. 1994
  • Elizabethkingia meningoseptica (King 1959) Kim et al. 2005

Elizabethkingia meningoseptica is a Gram negative rod shaped bacteria widely distributed in nature (e.g. fresh water, salt water, or soil). It may be normally present in fish and frogs but are not normally present in the human microflora. In 1959 American bacteriologist Elizabeth O. King (who isolated Kingella in 1960) at CDC Atlanta, was studying unclassified bacteria associated with meningitis in infants, when she isolated an organism (CDC group IIa) that she named Flavobacterium meningosepticum (Flavobacterium means "the yellow bacillus"; meningosepticum means "associated with meningitis and sepsis", not septic meningitis).[1] In 1994, it was reclassified in the genus Chryseobacterium and was named Chryseobacterium meningosepticum[2](chryseos = "golden" in Greek, so Chryseobacterium meaning again a golden/yellow rod similar to Flavobacterium). In 2005, a 16S rRNA phylogenetic tree of Chrysobacteria showed that C. meningosepticum along with C. miricola (which was reported to have been isolated from Russian space station Mir in 2001 and placed in the genus Chrysobacterium in 2003[3]) were close to each other but outside the tree of the rest of the Chryseobacteria and were then placed in a new genus Elizabethkingia named after the original discoverer of F. meningosepticum.[4]

Presence in plants[edit]

Two species of Elizabethkingia have recently been found to be abundant on the leaf and root surfaces of the tropical tree Gnetum gnemon, in Malaysia (Oh et al. 2012, in press) (Oh, Y., Kim M., et al. Adams J.M in press 2012. Distinctive bacterial communities in the rhizoplane of four tropical tree species. Microbial Ecology. In press. ). Its role in the biology of the plant is unknown. Several other species of tropical trees studied did not have Elizabethkingia present on their leaves or roots, suggesting a host-specific relationship with Gnetum.

Microbiology[edit]

It is a species of gram-negative, obligate aerobic, non-fastidious, non-spore forming, nonfermentative; nonmotile; slender; slightly curved rod; and catalase positive, oxidase positive, and indole-positive, OF glucose ox+/F-, urease negative (contrast E. miricila which is urease positive), mannitol positive, non-nitrate-reducing (Some of the atypical strains may be nitrite reducing[5]) saprophytic bacilli. It is gelatinase, esculin, ONPG & DNAse positive. Chryseobacteria (Flavobacteria) are in general indole positive in contrast to most other nonfermenters, however the reaction may be weak. E. meningoseptica grows well in regular incubators on blood agar and chocolate agar. Colonies are very pale yellow and may not be easily evident at 24 hours. Elizabethkingia meningoseptica strains either are not pigmented or produce a weak yellow nondiffusible pigment (e.g., the type strain; Bruun and Ursing, 1987), in contrast members of all Chryseobacterium species produce a yellow to orange nondiffusible flexirubin type pigment. Strains growing better at 40oC are mostly associated with invasive meningitis.[6] The greyish discoloration around the colonies on blood agar is due to the proteases & gelatinase. They grow poorly or not at all on MacConkey agar and are considered glucose oxidizers.[7] They do not grow on CNA (Colistin Nalidixic acid) agar because though they are resistant to colistin they are susceptible to quinolones like nalidixic acid.

E. meningoseptica may show colistin resistant and vancomycin sensitive/intermediate growth which is paradoxic for a gram negative bacteria, but resembles Burkholderia cepacia which is also a nonfermenter, does not grow on MacConkey agar in less than 3 days (grows late) and colistin resistant and vancomycin sensitive. These two can be distinguished by Indole test or Pyr test both of which should be clearly negative for Burkholderia cepacia and positive for E. meningoseptica. Automated bacterial identification system results should be observed with caution, especially when a patient with gram negative bacteremia does not improve with broad-spectrum antibiotic therapy, because several bacteria, including Aeromonas salmonicida (mistaken by ID32 GN [5]) and Sphingobacterium spp. (mistaken by Vitek 2 [8]), may be confused with this bacteria especially the atypical ones. However unlike many other Aeromonas species like Aeromonas hydrophilia, Aeromonas punctata, A. sulmonicida is indole negative which can help in distinguishing in case of doubt. An automated but so far relatively reliable Rapid NF plus system and API Zym systems use array of biochemical tests for a bettery of nonfermenters and other bacteria and can specifically identify E meningisepticum.


Infection[edit]

It has generally been reported to cause outbreaks of meningitis predominantly in premature newborns and infants in neonatal intensive care units of underdeveloped countries. Some of the outbreaks have been linked to sources like contaminated lipid stock bottles, contaminated venous catheter lines and nutritional solution, and tap water. It is also a rare cause of nosocomial pneumonia, endocarditis, postoperative bacteremia, and meningitis in immunocompromised adults . Only recently it has also been reported to cause soft tissue infection and sepsis in the immunocompetent[8] and a case report of a fatal necrotizing fasciitis in a diabetic patient.[9]

Antimicrobial susceptibility[edit]

This organism is usually multiresistant to antibiotics typically prescribed for treating gram-negative bacterial infections, including extended-spectrum ß-lactam agents (due to production by most strains of two betalactamases: one ESBL and one Class B Carbapenem-Hydrolyzing metallolactamase), aminoglycosides, tetracycline, and chloramphenicol. Though vancomycin has been used in the past, high MIC (16 µg/ml) for vancomycin lead to search for alternatives especially for meningitis. Presently ciprofloxacin, minocycline, trimethoprim-sulfamethoxazole, rifampin and novobiocin are being considered good alternatives. Most of these are classic drugs for gram positive bacteria and not routinely tested on gram negative bacteria).[10]

Predictors of poor outcome[edit]

Hypoalbuminemia, increased pulse rate at the onset of infection and presence of central venous line infection were associated with a poor outcome.[11]

Resource persons[edit]

In their 2006 meeting, International Committee on Systematics of Prokaryotes; Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria nominated J.-F. Bernardet and B. Bruun as two key authorities on this bacteria.[12]

Unambiguous synonyms[edit]

  • Flavobacterium meningosepticum King, 1959 (Approved Lists, 1980)
  • Chryseobacterium meningosepticum (King, 1959) Vandamme et al., 1994
  • Elizabethkingia meningoseptica (King, 1959) Kim et al., 2005

References[edit]

  1. ^ E. O. King (1959). "Studies on a group of previously unclassified bacteria associated with meningitis in infants". American Journal of Clinical Pathology 31 (3): 241–247. PMID 13637033. 
  2. ^ P. Vandamme, J. F. Bernardet, P. Segers, K. Kersters & B. Holmes (1994). "New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev.". International Journal of Systematic Bacteriology 44 (4): 827–831. doi:10.1099/00207713-44-4-827. 
  3. ^ Ying Li, Yoshiaki Kawamura, Nagatoshi Fujiwara, Takashi Naka, Hongsheng Liu, Xinxiang Huang, Kazuo Kobayashi & Takayuki Ezaki (2003). "Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir". Systematic and Applied Microbiology 26 (4): 523–528. doi:10.1078/072320203770865828. PMID 14666980. 
  4. ^ Kwang Kyu Kim, Myung Kyum Kim, Ju Hyoung Lim, Hye Yoon Park & Sung-Taik Lee (2005). "Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov". International Journal of Systematic and Evolutionary Microbiology 55 (3): 1287–1293. doi:10.1099/ijs.0.63541-0. 
  5. ^ a b Cheng-Hsun Chiu, Michael Waddingdon, Wu-Shiun Hsieh, David Greenberg, Paul C. Schreckenberger & Amy M. Carnahan (2000). "Atypical Chryseobacterium meningosepticum and meningitis and sepsis in newborns and the immunocompromised, Taiwan". Emerging Infectious Diseases 6 (5): 481–486. doi:10.3201/eid0605.000506. PMC 2627967. PMID 10998378. 
  6. ^ Jean-François Bernardet, Celia Hugo and Brita Bruun (2006). "The Genera Chryseobacterium and Elizabethkingia". The Prokaryotes. New York: Springer. pp. 638–676. doi:10.1007/0-387-30747-8_25. ISBN 978-0-387-25497-5. 
  7. ^ Koneman's Color Atlas and Textbook of Diagnostic Microbiology
  8. ^ a b Felipe Francisco Tuon, Luciana Campon, Gisels Duboc de Almeida & Ronaldo Cesar Gryschek (2007). "Chryseobacterium meningosepticum as a cause of cellulitis and sepsis in an immunocompetent patient". Journal of Medical Microbiology 56 (8): 1116–1117. doi:10.1099/jmm.0.47111-0. 
  9. ^ Ching-Chi Lee, Po-Lin Chen, Li-Rong Wang, Hsin-Chun Lee, Chia-Ming Chang, Nan-Yao Lee, Chi-Jung Wu, Hsin-I Shih & Wen-Chien Ko (2006). "Fatal case of community-acquired bacteremia and necrotizing fasciitis caused by Chryseobacterium meningosepticum: case report and review of the literature". Journal of Clinical Microbiology 44 (3): 1181–1183. doi:10.1128/JCM.44.3.1181-1183.2006. PMID 16517926. 
  10. ^ Pen-Yi Lin, Chishih Chu, Lin-Hui Su, Chung-Tsui Huang, Wen-Ya Chang & Cheng-Hsun Chiu (2004). "Clinical and microbiological analysis of bloodstream infections caused by Chryseobacterium meningosepticum in nonneonatal patients". Journal of Clinical Microbiology 42 (7): 3353–3355. doi:10.1128/JCM.42.7.3353-3355.2004. 
  11. ^ Po-Pin Hung, Yu-Hui Lin, Chin-Fu Lin, Meei-Fang Liu & Zhi-Yuan Shi (2008). "Chryseobacterium meningosepticum infection: antibiotic susceptibility and risk factors for mortality". Journal of Microbiology, Immunology and Infection 41: 137–144. 
  12. ^ Jean-François Bernardet (2006). "International Committee on Systematics of Prokaryotes. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria. Minutes of the meetings, 26 July 2005, San Francisco, CA, USA". International Journal of Systematic and Evolutionary Microbiology 56 (12): 2949–2951. doi:10.1099/ijs.0.64834-0. 

External links[edit]