Lesperance was born in Minneapolis and raised in Seattle. She creates art in various media but often employs the visual language of knitting, having once worked for Vogue Knitting as a pattern knitter. Citing inspiration from Bauhaus-era female weavers, the Pattern and Decoration Movement, and body-based feminist artists of the 1970s and 1980s, Lesperance’s gouache paintings on paper can be followed as patterns to recreate historic knit garments. She sources these historic garments from archival images and film footage of women involved in Direct Action protest, including women from: the Greenham Common Women's Peace Camp, the 1999 Seattle WTO protests, Earth First!, Occupy events, feminist-era protest events, and the feminist art canon. She frequently displays her paintings with her hand-knit textiles, which she says she hopes will “beckon a new wearer.” Additionally, Lesperance creates memorial paintings that she terms “death shrouds” for young women activists who have died while fighting for “causes greater than themselves,” these women include: Rachel Corrie, Beth “Horehound” O’Brien, Susana Chávez, Pippa Bacca, and Helen Thomas. In 2012, Lesperance presented a solo show at Frieze Art Fair New York in which she displayed a suite of seven paintings made in the memory of slain Italian activist and artist Pippa Bacca.
Lesperance has been represented by Ambach & Rice Gallery in Los Angeles, CA since 2010 and she is included in many public collections including: the Brooklyn Museum, the Museum of Arts and Design, the Portland Art Museum, the Bill and Melinda Gates Foundation, and the Kadist Art Foundation. In 2013, she was included in Phaidon Press’ Vitamin D2: New Perspectives in Drawing and the first International BiennaleOnline. Between 2000 and 2005, Lesperance was engaged in costume- and prop-making for a large-format photography project with collaborator Jeanine Oleson in which both women performed as the last women on earth. This project, entitled "Off the Grid," was represented by Monya Rowe Gallery, NYC, and was received by arts writers as an alternate depiction of pre-history for women wherein Lesperance and Oleson assumed "the mythical characters of prehistoric huntresses, earth goddesses, and pioneer women.", And like Lesperance's current activist sweater painting series, the work can be read as revisionist in its nature. "If this is theater," writes Julia Bryan-Wilson in ArtUS magazine, "it is theater as revelation.", Lesperance received her MFA in Visual Arts from Rutgers University in 1999.
- Rattemeyer, Christian (2013). ' 'Vitamin D2: New Perspectives in Drawing' '. Phaidon Press. ISBN 0714865281.
- "Art Splash: Ellen Lesperance - It's Never Over - Ambach and Rice - Los Angeles". Contemporaryartlinks.blogspot.com. 2013-02-16. Retrieved 2013-05-02.
- Virtua Design. "The Strong, Star-Bright Companions by Ellen Lesperance - Guernica / A Magazine of Art & Politics". Guernicamag.com. Retrieved 2013-05-02.
- "In Pictures - Frieze New York | AnOther". Anothermag.com. 2012-05-08. Retrieved 2013-05-02.
- Collections: Elizabeth A. Sackler Center for Feminist Art: Cardigan Worn by One Woman of the Boeing Five, Tried for Entering the Boeing Nuclear Missile Plant on September 27th, 1983, Sentenced to Fifteen Days in the King County Jail for Defending Life on Earth
- Milliard, Coline (2013-03-26). "Soon-to-Launch Online Biennale Includes 180 Artists, Will Charge for Admission | Artinfo". Sea.blouinartinfo.com. Retrieved 2013-05-02.
- Bessire, Mark (2006). ' 'Cryptozoology: Out of Time Place Scale' '. JRP|Ringier Press. ISBN 3905770075
- Koslow, Francis (2006). ' 'Exhibition Review, Tema Celeste Magazine, Issue 113:January/February
- Bryan-Wilson, Julia (2005). Exhibition Review, ArtUS Magazine, Issue 11:December