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EN, En or en can mean:
- En or N, the fourteenth letter of the Roman alphabet
- EN (cuneiform), the mark in Sumerian cuneiform script for a High Priest or Priestess meaning "lord" or "priest"
- En (Cyrillic) (Н, н), a letter of the Cyrillic alphabet, equivalent to the Roman letter "n"
- En (digraph), ‹en› used as a phoneme
- En (typography), a unit of width in typography, equivalent to half the height of a given font. (see also en dash)
- En (Lie algebra) - family of En Lie algebras, unique for n=5..8.
- En, abbreviation for the English language
- en, ISO 639-1 code for English language
- En, abbreviation for English studies, an academic subject
Science and technology
- Electronegativity, a measure of the ability of an atom or molecule to attract electrons in the context of a chemical bond
- En, En (Lie algebra), a type of algebra
- EN standards, European standards for products and services by European Committee for Standardization: see List of EN standards
- Engrailed (gene), a gene involved in early embryological development
- en, ethylenediamine, an organic compound with the formula C2H8N2
- EN, exanewton, an SI unit of force: 1018 newtons
- Erythema nodosum, an inflammation of the fat cells under the skin
- Electroless nickel plating, a chemical technique for nickel-alloy plating
- EN, FIPS 10-4 code for Estonia
- EN, obsolete NATO country code for Estonia (now EST)
- EN postcode area, an area of London
- Einstürzende Neubauten, a German industrial music group
- En or Ann, an antagonist from the Makaiju episodes of the Sailor Moon anime series
- Eastern National, a US non-profit organization providing support to the National Park Service
- English Nature, former UK government agency responsible for nature conservation in England
- Envirolink Northwest, an environmental organisation in north-west England
- Escola Naval (translated from Portuguese: Navy College), is the academy of the Navy of Brazil
- Ensign (rank), a military rank
- En, direct transliteration from Japanese of Yen, the unit of Japanese currency
- Enrolled Nurse, an Australian term for a licensed practical nurse
- Empty netter or empty net goal, a type of goal in ice hockey
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