Lysin

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Not to be confused with Lysine.
For other uses, see Lysin (disambiguation).
Lysozyme-like phage lysin
Crystal structure of the modular CPL-1 endolysin complexed with a peptidoglycan analogue.jpg
Crystal structure of the modular CPL-1 endolysin from Streptococcus phage Cp-1 complexed with a peptidoglycan analogue. PDB entry 2j8g.[1]
Identifiers
EC number 3.2.1.17
CAS number 9001-63-2
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

Lysins, also known as endolysins or murein hydrolases, are hydrolytic enzymes produced by bacteriophages in order to cleave the host's cell wall during the final stage of the lytic cycle. Lysins are highly evolved enzymes that are able to target one of the five bonds in peptidoglycan (murein), the main component of bacterial cell walls, which allows the release of progeny virions from the lysed cell. These enzymes are being used as antibacterial agents due to their high effectiveness and specificity in comparison with antibiotics, which are susceptible to bacterial resistance.[2]

Lysins are not synthesized by some small single-stranded DNA and RNA phages which produce membrane proteins that activate the host's autolytic mechanisms.[3]

Structure[edit]

Double-stranded DNA phage lysins tend to lie within the 25 to 40 kDa range in terms of size. A notable exception is the streptococcal PlyC endolysin, which is 114 kDa. PlyC is not only the biggest and most potent lysin, but also structurally unique since it is composed of two different gene products, PlyCA and PlyCB, with a ratio of eight PlyCB subunits for each PlyCA in its active conformation.[4] All other lysins are monomeric and comprise two domains separated by a short linker region. The N-terminal domain catalyses the hydrolysis of peptidoglycan whereas the C-terminal domain binds to the cell wall substrate.

Catalytic domain[edit]

The catalytic domain is responsible for the cleavage of peptidoglycan bonds. Functionally, five types of lysin catalytic domain can be distinguished:

Peptidoglycan consists of cross-linked amino acids and sugars which form alternating amino sugars: N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM). Endo-β-N-acetylglucosaminidase lysins cleave NAGs while N-acetylmuramidase lysins (lysozyme-like lysins) cleave NAMs. Endopeptidase lysins cleave any of the peptide bonds between amino acids, whereas N-acetylmuramoyl-l-alanine amidase lysins (or simply amidase lysins) hydrolyze the amide bond between the sugar and the amino acid moieties. Finally, the recently discovered γ-d-glutaminyl-l-lysine endopeptidase lysins cleave the gamma bond between D-glutamine and L-lysine residues.

Usually, two or more different catalytic domains are linked to a single cell-binding domain. This is typical in many staphylococcal lysins as well as the streptococcal PlyC holoenzyme, which contains two catalytic domains.[5][4] Catalytic domains are highly conserved in phage lysins of the same class.[2]

Cell-binding domain[edit]

The cell-binding domain (CBD) binds to a specific substrate found in the host bacterium's cell wall, usually a carbohydrate. In contrast to the catalytic domain, the cell-binding domain is variable, which allows a great specificity and decreases bacterial resistance.[6] Binding affinity to the cell wall substrate tends to be high, possibly so as to limit amount of free enzyme released, which would lyse other bacteria preventing the virus from reproducing.[7]

Evolution[edit]

It has been proposed that the main mechanism of evolution in phage lysins is the exchange of modular units, a process by which different catalytic and cell-binding domains have been swapped between lysins, which would have resulted in new combinations of both bacterial binding and catalytic specificities.[8]

Mode of action[edit]

The lysin catalytic domain digests peptidoglycan locally at a high rate, which causes holes in the cell wall. Since the cross-linked peptidoglycan cell wall is the only mechanism that prevents the spontaneous burst of bacterial cells due to the high internal pressure (3 to 5 atmospheres), enzymatic digestion by lysins irreversibly causes hypotonic lysis. Theoretically, due to the catalytic properties of phage lysins, a single enzyme would be sufficient to kill the host bacterium by cleaving the necessary number of bonds, even though this has yet to be proven.[2] The work by Loessner et al suggests that cleavage is typically achieved by the joint action of multiple lysin molecules at a local region of the host's cell wall.[7] The high binding affinity to the cell wall substrate (close to that of IgG for its substrate) of each lysin appear to be reason why multiple molecules are required, since every lysin binds so tightly to the cell wall that it can't break enough bonds to cause lysis by itself.[7]

In order to reach the cell wall, phage lysins have to cross the cell membrane. However, they lack a signal peptide that would allow them to do so. In order to solve such a problem, phage viruses synthesize another protein called holin which binds to the cell membrane and makes holes in it (hence its name), allowing lysins to reach the peptidoglycan matrix. The prototypical holin is the lambda phage S protein, which assists the lambda phage R protein (lysin). All holins embed themselves in the cell membrane and contain at least two transmembrane helical domains. The hole making process is thought to be achieved by holin oligomerization at a specific moment when progeny virions are set to be released.[3][9]

Efficacy[edit]

Phage lysins are generally species or subspecies specific, which means that the are only effective against bacteria from which they were produced. While some lysins only act upon the cell walls of several bacterial phylotypes, some broad-spectrum lysins have been found.[10] Similarly, some thermostable lysins are known, which makes them easier to use in biotechnology.[11] Regarding their use as antibacterial agents, lysins have been found effective exclusively against Gram-positive bacteria, since Gram-negative bacteria possess an outer membrame that prevents extracellular lysin molecules from digesting peptidoglycan.[2]

Immune response[edit]

One of the most problematic aspects of the use phage lysins as antimicrobial agents is the potential immunogenicity of these enzymes. Unlike most antibiotics, proteins are prone to antibody recognition and binding, which means that lysins could be ineffective when treating bacterial infections or even dangerous, potentially leading to a systemic immune response or a cytokine storm. Nonetheless, experimental data from immunologically-rich rabbit serum showed that hyperimmune serum slows down but does not block the activity of pneumococcal lysin Cpl-1.[12]

Antimicrobial use[edit]

Phage lysins have been successfully tested in animal models to control pathogenic antibiotic-resistant bacteria found on mucous membranes and in blood. The main advantage of lysins compared to antibiotics is not only the low bacterial resistance but also the high specificity towards the target pathogen, as well as the innocuousness towards the host patient and its normal bacterial flora.[2]

See also[edit]

References[edit]

  1. ^ Pérez-Dorado I, Campillo NE, Monterroso B, Hesek D, Lee M, Páez JA, García P, Martínez-Ripoll M, García JL, Mobashery S, Menéndez M, Hermoso JA (August 2007). "Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1". J. Biol. Chem. 282 (34): 24990–9. doi:10.1074/jbc.M704317200. PMID 17581815. 
  2. ^ a b c d e Fischetti VA (October 2008). "Bacteriophage lysins as effective antibacterials". Curr. Opin. Microbiol. 11 (5): 393–400. doi:10.1016/j.mib.2008.09.012. PMC 2597892. PMID 18824123. 
  3. ^ a b Young R (September 1992). "Bacteriophage lysis: mechanism and regulation". Microbiol. Rev. 56 (3): 430–81. PMC 372879. PMID 1406491. 
  4. ^ a b McGowan S, Buckle AM, Mitchell MS, Hoopes JT, Gallagher DT, Heselpoth RD, Shen Y, Reboul CF, Law RH, Fischetti VA, Whisstock JC, Nelson DC (July 2012). "X-ray crystal structure of the streptococcal specific phage lysin PlyC". Proc. Natl. Acad. Sci. U.S.A. 109 (31): 12752–7. Bibcode:2012PNAS..10912752M. doi:10.1073/pnas.1208424109. PMC 3412044. PMID 22807482. 
  5. ^ Navarre WW, Ton-That H, Faull KF, Schneewind O (May 1999). "Multiple enzymatic activities of the murein hydrolase from staphylococcal phage phi11. Identification of a D-alanyl-glycine endopeptidase activity". J. Biol. Chem. 274 (22): 15847–56. doi:10.1074/jbc.274.22.15847. PMID 10336488. 
  6. ^ García E, García JL, García P, Arrarás A, Sánchez-Puelles JM, López R (February 1988). "Molecular evolution of lytic enzymes of Streptococcus pneumoniae and its bacteriophages". Proc. Natl. Acad. Sci. U.S.A. 85 (3): 914–8. Bibcode:1988PNAS...85..914G. doi:10.1073/pnas.85.3.914. JSTOR 31364. PMC 279667. PMID 3422470. 
  7. ^ a b c Loessner MJ, Kramer K, Ebel F, Scherer S (April 2002). "C-terminal domains of Listeria monocytogenes bacteriophage murein hydrolases determine specific recognition and high-affinity binding to bacterial cell wall carbohydrates". Mol. Microbiol. 44 (2): 335–49. doi:10.1046/j.1365-2958.2002.02889.x. PMID 11972774. 
  8. ^ García P, García JL, García E, Sánchez-Puelles JM, López R (January 1990). "Modular organization of the lytic enzymes of Streptococcus pneumoniae and its bacteriophages". Gene 86 (1): 81–8. doi:10.1016/0378-1119(90)90116-9. PMID 2311937. 
  9. ^ Wang IN, Smith DL, Young R (2000). "Holins: the protein clocks of bacteriophage infections". Annu. Rev. Microbiol. 54: 799–825. doi:10.1146/annurev.micro.54.1.799. PMID 11018145. 
  10. ^ Yoong P, Schuch R, Nelson D, Fischetti VA (July 2004). "Identification of a broadly active phage lytic enzyme with lethal activity against antibiotic-resistant Enterococcus faecalis and Enterococcus faecium". J. Bacteriol. 186 (14): 4808–12. doi:10.1128/JB.186.14.4808-4812.2004. PMC 438584. PMID 15231813. 
  11. ^ Plotka M, Kaczorowska AK, Stefanska A, Morzywolek A, Fridjonsson OH, Dunin-Horkawicz S, Kozlowski L, Hreggvidsson GO, Kristjansson JK, Dabrowski S, Bujnicki JM, Kaczorowski T (2014). "Novel Highly Thermostable Endolysin from Thermus scotoductus MAT2119 Bacteriophage Ph2119 with Amino Acid Sequence Similarity to Eukaryotic Peptidoglycan Recognition Proteins". Appl. Environ. Microbiol. 80 (3): 886–95. doi:10.1128/AEM.03074-13. PMC 3911187. PMID 24271162. 
  12. ^ Loeffler JM, Djurkovic S, Fischetti VA (November 2003). "Phage lytic enzyme Cpl-1 as a novel antimicrobial for pneumococcal bacteremia". Infect. Immun. 71 (11): 6199–204. doi:10.1128/IAI.71.11.6199-6204.2003. PMC 219578. PMID 14573637.