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|Citrobacter freundii, one member of the family|
The Enterobacteriaceae is a large family of Gram-negative bacteria that includes, along with many harmless symbionts, many of the more familiar pathogens, such as Salmonella, Escherichia coli, Yersinia pestis, Klebsiella and Shigella. Other disease-causing bacteria in this family include Proteus, Enterobacter, Serratia, and Citrobacter. This family is the only representative in the order Enterobacteriales of the class Gammaproteobacteria in the phylum Proteobacteria. Phylogenetically, in the Enterobacteriales, several peptidoglycan-less insect endosymbionts form a sister clade to the Enterobacteriaceae, but as they are not validly described, this group is not officially a taxon; examples of these species are Sodalis, Buchnera, Wigglesworthia, Baumannia and Blochmannia, but not formers rickettsias. Members of the Enterobacteriaceae can be trivially referred to as enterobacteria, as several members live in the intestines of animals. In fact, the etymology of the family is enterobacterium with the suffix to designate a family (aceae) — not after the genus Enterobacter (which would be "Enterobacteraceae")— and the type genus is Escherichia.
Members of the Enterobacteriaceae are rod-shaped, and are typically 1-5 μm in length. Like other proteobacteria, enterobacteria have Gram-negative stains, and they are facultative anaerobes, fermenting sugars to produce lactic acid and various other end products. Most also reduce nitrate to nitrite, although exceptions exist (e.g. Photorhabdus). Unlike most similar bacteria, enterobacteria generally lack cytochrome C oxidase, although there are exceptions (e.g. Plesiomonas shigelloides). Most have many flagella used to move about, but a few genera are nonmotile. They are not spore-forming. Catalase reactions vary among Enterobacteriaceae.
Many members of this family are a normal part of the gut flora found in the intestines of humans and other animals, while others are found in water or soil, or are parasites on a variety of different animals and plants. Escherichia coli is one of the most important model organisms, and its genetics and biochemistry have been closely studied.
Most members of Enterobacteriaceae have peritrichous, type I fimbriae involved in the adhesion of the bacterial cells to their hosts. Some enterobacteria produce endotoxins. Endotoxins reside in the cell cytoplasm and are released when the cell dies and the cell wall disintegrates. Some members of the Enterobacteriaceae family produce a systemic infection into the blood stream when all the dead bacterial cells release their endotoxins. This is known as endotoxic shock, and can be rapidly fatal.
- Phenol red
- Tryptone broth
- Phenylalanine agar for detection of production of deaminase, which converts phenylalanine to phenylpyruvic acid
- Methyl red or Voges-Proskauer tests depend on the digestion of glucose. The methyl red tests for acid endproducts. The Voges Proskauer tests for the production of acetylmethylcarbinol.
- Catalase test on nutrient agar tests for the production of catalase enzyme, which splits hydrogen peroxide and releases oxygen gas.
- Oxidase test on nutrient agar tests for the production of the enzyme oxidase, which reacts with an aromatic amine to produce a purple color.
- Nutrient gelatin tests to detect activity of the enzyme gelatinase.
In a clinical setting, three species make up 80 to 95% of all isolates identified. These are Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis.
Antibiotic resistance 
Several Enterobacteriacea strains have been isolated which are resistant to antibiotics including carbapenem, which are often claimed as "the last line of antibiotic defense" against resistant organisms. For instance, some Klebsiella pneumonia strains are carbapenem resistant.
The following inexhaustive list details bacterial genera classified as members of Enterobacteriaceae.
- Erwinia, e.g. Erwinia amylovora, Erwinia tracheiphila, Erwinia carotovora, etc.
- Escherichia, e.g. Escherichia coli
- Klebsiella, e.g. Klebsiella pneumoniae
- Pectobacterium see Erwinia
- Candidatus Phlomobacter
- Photorhabdus, e.g. Photorhabdus luminescens
- Plesiomonas, e.g. Plesiomonas shigelloides
- Proteus, e.g. Proteus vulgaris
- Serratia, e.g. Serratia marcescens
- Yersinia, e.g. Yersinia pestis
- George M. Garrity, ed. (July 26, 2005) [1984 (Williams & Wilkins)]. The Gammaproteobacteria. Bergey's Manual of Systematic Bacteriology 2B (2nd ed.). New York: Springer. p. 1108. ISBN 978-0-387-24144-9. British Library no. GBA561951.
- Williams, K. P.; Gillespie, J. J.; Sobral, B. W. S.; Nordberg, E. K.; Snyder, E. E.; Shallom, J. M.; Dickerman, A. W. (2010). "Phylogeny of Gammaproteobacteria". Journal of Bacteriology 192 (9): 2305–2314. doi:10.1128/JB.01480-09. PMC 2863478. PMID 20207755. Text "20207755" ignored (help)
- "Dorlands Medical Dictionary:Enterobacteriaceae".
- MacFaddin, Jean F. Biochemical Tests for Identification of Medical Bacteria. Williams & Wilkins, 1980, p 441.
- Centers for Disease Control and Prevention - Klebsiella Quotation: "Increasingly, Klebsiella bacteria have developed antimicrobial resistance, most recently to the class of antibiotics known as carbapenems."
- Enterobacteriaceae genomes and related information at PATRIC, a Bioinformatics Resource Center funded by NIAID
- Evaluation of new computer-enhanced identification program for microorganisms: adaptation of BioBASE for identification of members of the family Enterobacteriaceae 
- Brown, A.E. (2009). Benson's microbiological applications: laboratory manual in general microbiology. New York: McGraw- Hill.