Evans blue

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For the Canadian rock band, see Evans Blue.
Evans blue
Evans Blue.svg
Space-filling model of the Evans blue molecule, sodium salt
IUPAC name
tetrasodium (6E,6'E)-6,6-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1E)hydrazin-2-yl-1-ylidene]bis(4-amino-5-oxo-5,6-dihydronaphthalene-1,3-disulfonate)
314-13-6 YesY
ChEMBL ChEMBL1200712 N
Jmol-3D images Image
KEGG C19422 N
MeSH Evans+blue
PubChem 6321418
Molar mass 960.81 g·mol−1
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 N verify (what isYesY/N?)
Infobox references

T-1824 or Evans Blue, often incorrectly rendered as Evan's Blue, is an azo dye which has a very high affinity for serum albumin. Because of this, it can be useful in physiology in estimating the proportion of body water contained in blood plasma.[1]

Evans blue dye has been used as a viability assay on the basis of its penetration into non-viable cells, although the method is subject to error because it assumes that damaged or otherwise altered cells are not capable of repair and therefore are not viable.[2]

Evans blue is also used to assess the permeability of the blood–brain barrier to macromolecules. Because serum albumin cannot cross the barrier, and virtually all Evans Blue is bound to albumin, normally the neural tissue remains unstained.[3] When the BBB has been compromised, albumin-bound Evans blue enters the CNS. It fluoresces with excitation peaks at 470 and 540 nm and an emission peak at 680 nm. [4]

Evans blue is pharmacologically active, acting as a negative allosteric modulator of the AMPA and kainate receptors and as an inhibitor of vesicular glutamate transporters.[citation needed]

It was named after Herbert McLean Evans, an American chemist.


  1. ^ Physiology at MCG 7/7ch02/7ch02p17
  2. ^ Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)" (HTML). European Journal of Phycology 34 (1): 43–52. doi:10.1080/09670269910001736072. 
  3. ^ Hawkins BT, Egleton RD (2006). "Fluorescence imaging of blood–brain barrier disruption". Journal of Neuroscience Methods 151 (2): 262–7. doi:10.1016/j.jneumeth.2005.08.006. PMID 16181683. 
  4. ^ Hed J, Dahlgren C, and Rundquist, I (1983). "A Simple Fluorescence Technique to Stain the Plasma Membrane of Human Neutrophils". Histochemistry 79 (1): 105–10. doi:10.1007/BF00494347. PMID 6196326. 

External links[edit]

  • el-Sayed H, Goodall S, Hainsworth R (1995). "Re-evaluation of Evans blue dye dilution method of plasma volume measurement". Clin Lab Haematol 17 (2): 189–94. PMID 8536425.