Exoribonuclease

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Reaction diagrams for both hydrolytic (left) and phosphorolytic (right) 3'-5' exoribonuclease degradation of RNA.

An exoribonuclease is an exonuclease ribonuclease, which are enzymes that degrade RNA by removing terminal nucleotides from either the 5' end or the 3' end of the RNA molecule. Enzymes that remove nucleotides from the 5' end are called 5'-3' exoribonucleases, and enzymes that remove nucleotides from the 3' end are called 3'-5' exoribonucleases.

Exoribonucleases can use either water to cleave the nucleotide-nucleotide bond (which is called hydrolytic activity) or inorganic phosphate (which is called phosphorolytic activity). Hydrolytic exoribonucleases are classified under EC number 3.1 and phosphorolytic exoribonucleases under EC number 2.7.7. As the phosphorolytic enzymes use inorganic phosphate to cleave bonds they release nucleotide disphosphates), whereas the hydrolytic enzymes (which use water) release nucleotide monosphosphates).

Exoribonucleases exist in all kingdoms of life, the bacteria, archaea, and eukaryotes. Exoribonucleases are involved in the degradation of many different RNA species, including messenger RNA, transfer RNA, ribosomal RNA and miRNA. Exoribonucleases can be single proteins (like RNase D or RNase PH) but also can be complexes of multiple proteins, like the exosome complex (in which four of the major exoribonuclease families are represented).

Major families[edit]

Family Example members Distribution Catalytic activity
RNR RNase R Most bacteria, all eukaryotes 3'-5' hydrolytic
RNase II
Rrp44
DEDD RNase D Certain bacteria, all eukaryotes 3'-5' hydrolytic
RNase T
PM/Scl-100
Oligoribonuclease
RBN RNase BN Certain bacteria 3'-5' hydrolytic
PDX PNPase All kingdoms 3'-5' phosphorolytic
PM/Scl-75
RNase PH
RRP4 Rrp4 All eukaryotes, most archaea 3'-5' hydrolytic
5PX Exoribonuclease I All eukaryotes 5'-3' hydrolytic
Exoribonuclease II

See also[edit]

References[edit]

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