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FCI may refer to:
- Facility condition index, a benchmark to compare the relative condition of a group of facilities
- Federal Correctional Institution
- Fédération Cynologique Internationale, international dog breed standards organization
- Federazione Ciclistica Italiana, the national governing body of cycle racing in Italy
- Interoceanic Railway of Mexico (Ferrocarril Interoceánico de México), one of the railroad companies of Mexico, now part of the Ferrocarriles Nacionales de México
- Food Corporation of India
- The Francis Crick Institute, a biomedical research centre to be located in London, United Kingdom
- French Culinary Institute, a culinary school in New York City
- Frodus (a.k.a. Frodus Conglomerate International, FCI), a 1990s post-hardcore band
- Fujisankei Communications International, the American subsidiary of the Fujisankei Communications Group and a former video game company
- Full configuration interaction
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