FISSEQ

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FISSEQ is an abbreviation for "Fluorescent in situ sequencing", a method of sequencing DNA by the use of polonies and cycles of fluorescent deoxyribonucleotide triphosphate (dNTP) incorporation.[1] Although FISSEQ does not generate as many reads as RNA-Seq, FISSEQ mainly detects genes that characterize cell type and function. This means that FISSEQ might be a means to identify cell types in situ based on gene expression profiles.[2]

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References[edit]

  1. ^ Mitra RD, Shendure J, Olejnik J, Edyta-Krzymanska-Olejnik, Church GM (2003). "Fluorescent in situ sequencing on polymerase colonies". Analytical Biochemistry 320 (1): 55–65. PMID 12895469. 
  2. ^ Lee JH, Daugharthy ER, Scheiman J, Kalhor R, Yang JL, Ferrante TC, Terry R, Jeanty SS, Li C, Amamoto R, Peters DT, Turczyk BM, Marblestone AH, Inverso SA, Bernard A, Mali P, Rios X, Aach J, Church GM (2014). "Highly multiplexed subcellular RNA sequencing in situ". Science (journal) 343 (6177): 1360–1363. doi:10.1126/science.1250212. PMID 24578530.