FYVE domain

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FYVE zinc finger
1joc.gif
Early endosome antigen 1 (EEA1) dimer with lipids.[1]
Identifiers
Symbol FYVE
Pfam PF01363
InterPro IPR000306
PROSITE PDOC50178
SCOP 1vfy
SUPERFAMILY 1vfy
OPM superfamily 62
OPM protein 1vfy
CDD cd00065

In molecular biology the FYVE zinc finger domain is named after the four cysteine-rich proteins: Fab 1 (yeast orthologue of PIKfyve), YOTB, Vac 1 (vesicle transport protein), and EEA1, in which it has been found. FYVE domains bind Phosphatidylinositol 3-phosphate,[2] in a way dependent on its metal ion coordination and basic amino acids. The FYVE domain inserts into cell membranes in a pH dependent manner.[3] The FYVE domain has been connected to vacuolar protein sorting and endosome function.[4]

Structure[edit]

The FYVE domain is composed of two small beta hairpins (or zinc knuckles) followed by an alpha helix.[5] The FYVE finger binds two zinc ions. The FYVE finger has eight potential zinc coordinating cysteine positions and is characterized by having basic amino acids around the cysteines. Many members of this family also include two histidines in a sequence motif:

R+HHC+XCG, where + represents a charged residue and X any residue

The FYVE finger is structurally similar to the RING domain and the PHD finger.

Examples[edit]

The following is a list of human proteins containing this domain:

References[edit]

  1. ^ Dumas JJ, Merithew E, Sudharshan E, et al. (November 2001). "Multivalent endosome targeting by homodimeric EEA1". Mol. Cell 8 (5): 947–58. doi:10.1016/S1097-2765(01)00385-9. PMID 11741531. 
  2. ^ Gaullier JM, Simonsen A, D'Arrigo A, Bremnes B, Stenmark H, Aasland R (July 1998). "FYVE fingers bind PtdIns(3)P". Nature 394 (6692): 432–3. doi:10.1038/28767. PMID 9697764. 
  3. ^ He J, Vora M, Haney RM, et al. (September 2009). "Membrane insertion of the FYVE domain is modulated by pH". Proteins 76 (4): 852–60. doi:10.1002/prot.22392. PMC 2909462. PMID 19296456. 
  4. ^ Leevers SJ, Vanhaesebroeck B, Waterfield MD (April 1999). "Signalling through phosphoinositide 3-kinases: the lipids take centre stage". Curr. Opin. Cell Biol. 11 (2): 219–25. doi:10.1016/S0955-0674(99)80029-5. PMID 10209156. 
  5. ^ Misra S, Hurley JH (May 1999). "Crystal structure of a phosphatidylinositol 3-phosphate-specific membrane-targeting motif, the FYVE domain of Vps27p". Cell 97 (5): 657–66. doi:10.1016/S0092-8674(00)80776-X. PMID 10367894. 

Further reading[edit]

  • Stenmark H, Aasland R, Toh BH, D'Arrigo A (September 1996). "Endosomal localization of the autoantigen EEA1 is mediated by a zinc-binding FYVE finger". J. Biol. Chem. 271 (39): 24048–54. PMID 8798641.  edit
  • Stenmark H, Aasland R (December 1999). "FYVE-finger proteins--effectors of an inositol lipid". J. Cell. Sci. 112 (Pt 23): 4175–83. PMID 10564636.  edit