Florida State Road 121
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| Route information | ||||
| Maintained by FDOT | ||||
| Major junctions | ||||
| South end: |    US 19 / US 98 / SR 55 in Lebanon Jct. |
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  US 41 / SR 45 in Williston    US 27 Alt. / SR 500 in Williston US 27 / SR 500 in Williston  I-75 / SR 93 near Gainesville   US 441 / SR 20 / SR 25 in Gainesville  I-10 / SR 8 in Macclenny  US 90 / SR 10 in Macclenny |
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| North end: |   SR 23 / SR 121 near Stokesville, GA |
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| Highway system | ||||
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→State Road 121 is a major state highway that runs north and south in northern Florida. The road is actually part of a long multi-state route that also goes through Georgia and South Carolina. Some see it as a de facto auxiliary route from U.S. Route 21 in Rock Hill, South Carolina. Efforts to have the road upgraded to such status have failed however.
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Route description[edit]
Beginning in Lebanon Junction at an at-grade interchange with US 19-98 and Levy County Road 336 (LCR 336 joins unsigned with US 19-98 north through Otter Creek), State Road 121 runs through Goethe State Forest where it turns east and intersects with Levy CR 337. The road makes a sharp left curve to the northeast where it runs through desolate farmland; the only resemblance to a major intersection is Levy CR 326. Within the city of Williston, SR 121 overlaps Levy CR 316, until the intersection with US 41. It then joins US 41 as LCR 316 continues eastward, which then joins the east end of ALT US 27, and then the US 27-41 concurrency before breaking away to the northeast again, taking the DeSoto Trail away from US 41.
North of Levy CR 335, SR 121 hugs the west side of the Levy-Marion County Line, but never crosses it, choosing to enter Alachua County instead. Immediately after the interchange with Interstate 75(Exit 382), SR 121 makes a sharp left onto Northwest 34th Street as SR 331 becomes the designation for Williston Road. As it crosses SR 24 it borders the University of Florida to the east until crossing SR 26A and then SR 26. North of US 441, the DeSoto Trail breaks away.
After the intersection with CR 236 in Santa Fe, SR 121 is carried along bridges across the former Seaboard Coast Line railroad line to Crystal River Nuclear Power Plant and Santa Fe River in Worthington Springs, where it intersects with SR 18.
Briefly running parallel to SR 100 in Lake Butler, it intersects with SR 100 as it continues northeast. The road also intersects SR 16 in Raiford, and Interstate 10(Exit 335) and US 90 in Macclenny close to SR 228 before crossing the Georgia border on its way to South Carolina.
Major intersections[edit]
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This section contains a table that is missing mileposts for one or more junctions. Please help by adding the missing mileposts. |
| County | Location | Mile | Destinations | Notes | |
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| Levy | Lebanon Junction | 0.00 |  US 19 / US 98 / SR 55 / CR 336 |
Southern terminus | |
 CR 337 |
Near Goethe State Forest | ||||
| Morriston |  CR 326 |
Bi-County extension of SR 326 | |||
| Williston | US 41 / SR 45 |
Begin US 41 overlap | |||
| US 27 Alt. / SR 500 |
Begin Alt-US 27 overlap | ||||
| US 27 / SR 500 |
Southern terminus of Alt-US 27 Begin US 27 overlap |
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| US 27 / US 41 / SR 45 |
End US 27-41 overlap | ||||
| Alachua | I-75 / SR 93 |
Exit 382 on I-75 | |||
| Gainesville |  SR 331 |
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 SR 24 |
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 SR 26A |
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 SR 26 |
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 SR 222 |
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| US 441 / SR 20 / SR 25 |
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| LaCrosse |  SR 235 |
South end of SR 235 overlap | |||
| SR 235 |
North end of SR 235 overlap | ||||
| Santa Fe River Crossing | |||||
| Union | Worthington Springs |  SR 18 |
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| Lake Butler |  SR 231 |
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 SR 100 |
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| Raiford |  SR 16 |
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| Baker | Macclenny | I-10 / SR 8 |
Exit 335 on I-10 | ||
| US 90 / SR 10 |
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| SR 23 / SR 121, Stokesville |
Northern terminus at Florida-Georgia Border |
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Alachua County Road 121A[edit]
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| Location: | LaCrosse |
Alachua County Road 121A is the sole suffixed alternate of SR 121 that exists within the State of Florida. It is a former segment of SR 121 that exists entirely in LaCrosse. The route is unmarked, and runs along the east side of SR 121 south of downtown to halfway between the SR 121/235 overlap across from the LaCrosse Post Office.
