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GDB may refer to:
- GDB Human Genome Database, a community curated collection of human genomic data
- GNU Debugger, the standard debugger for the GNU operating system
- God, the Devil and Bob, an animated sitcom which premiered on NBC
- Goombay Dance Band, a German based band of the 1970s created by Oliver Bendt
- Guide Dogs for the Blind, a guide dog school located in the United States
- .gdb, a filename extension used by ArcGIS Geodatabase folders
- Puerto Rico Government Development Bank, the intragovernmental bank of Puerto Rico
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