The aim of the game is to be the last player riding. Cycles can be boosted with a limited turbo. There are various gameplay styles, including switching the gamplay from "booster" to "wall accel." to "both". "Booster" has an extra button for boosting, in addition to the standard left, right, glance left, glance right buttons, while "Wall ride" increases a player's light cycle's speed automatically depending on how close they are to an opponent's wall. "Both" incorporates both options for increasing the player's light cycle's speed.
There are several arena sizes that can be selected, from "tiny" (which is best for two players on normal speed or people practicing their reflexes with 3 "mcp himself" difficulty-level bots on "crazy" speed) to "vast".
The game is played using the keyboard to control the vehicle and the mouse to control the camera position, players can out ride competitors across the geometric grid.
The game's soundtrack features the track Revenge of the Cats, which was composed and performed by Finnish musician Peter Hajba, who is also known by his demoscene nickname Skaven.
In 2011 GLTron has been ported to the Symbian platform for phones that have support for OpenGL ES by Ronen K.