Barker was also one of the earliest photographers to visit the state of Florida. At the time, photography in Florida was challenging, as much of the state remained undeveloped, which meant photographers needed to carry their bulky equipment through the state's wetlands and subtropical jungles, as well as deal with delicate film in hot and humid conditions. Barker spent nearly four years (on and off), from 1886 to 1890, documenting much of northern and central Florida.
When he died in 1894, he was described as "the eminent photographer of Niagara Falls". His works were acquired by Underwood & Underwood of Washington, D.C.