|Professor George Brownlee|
|Born||George Gow Brownlee
13 January 1942
|Institutions||University of Cambridge
University of Oxford
Laboratory of Molecular Biology
Sir William Dunn School of Pathology
|Alma mater||Emmanuel College, Cambridge|
|Thesis||Nucleotide sequences in the low molecular weight ribosomal ribonucleic acid of Escherichia coli (1967)|
|Doctoral advisor||Frederick Sanger|
|Notable awards||Fellow of the Royal Society (1987)
Brownlee cloned and expressed human clotting factor IX, providing a recombinant source of this protein for Haemophilia B patients who had previously relied on the hazardous blood-derived product.
With Merlin Crossley he helped discover the two sets of genetic mutations that were preventing two key proteins from attaching to the DNA of people with a rare and unusual form of Haemophilia B - Haemophilia B Leyden - where sufferers experience episodes of excessive bleeding in childhood but have few bleeding problems after puberty. This lack of protein attachment to the DNA was thereby turning off the gene that produces clotting factor IX, which prevents excessive bleeding.
Awards and honours
|“||Distinguished for his work on the sequences of nucleic acids and their biological implications. He contributed to the development of methods using 32P-labelling and two-dimensional fractionation techniques, which greatly accelerated the early RNA sequencing. He used these methods to determine the sequence of the 5S ribosomal RNA, at that time the largest nucleic acid to be sequenced. He used fingerprint analysis of messenger RNA to demonstrate that immunoglobulin V- and C-regions were not discontinuous at the messenger RNA level, and early analysis of messenger RNA to identify a precursor for light chain synthesis. Parallel studies on globin messenger RNA demonstrated important features of eucaryotic translation. More recently he has developed faster methods for RNA sequencing and has applied them to transfer RNAs and ovalbumin messenger RNA. He also studied the DNA sequence of the ovalbumin gene and its insertion sequences. He determined the nucleotide sequence of the multiple gene coding for the 5S RNA in Xenopus laevis and showed that the coding regions alternated with a repetitious region and a "pseudogene" that had a sequence homologous with part of the 5S region.||”|
- "BROWNLEE, Prof. George Gow". Who's Who 2013, A & C Black, an imprint of Bloomsbury Publishing plc, 2013; online edn, Oxford University Press.(subscription required)
- "Library and Archive Catalogue Brownlee". London: The Royal Society. Retrieved 2013-11-11.
- "Professor George Brownlee, Lincoln College, Oxford". Archived from the original on 2013-12-17.
- George Brownlee from the Scopus bibliographic database.
- Rao, Z.; Handford, P.; Mayhew, M.; Knott, V.; Brownlee, G. G.; Stuart, D. (1995). "The structure of a Ca(2+)-binding epidermal growth factor-like domain: Its role in protein-protein interactions". Cell 82 (1): 131–141. doi:10.1016/0092-8674(95)90059-4. PMID 7606779.
- Caton, A. J.; Brownlee, G. G.; Yewdell, J. W.; Gerhard, W. (1982). "The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype)". Cell 31 (2 Pt 1): 417–427. doi:10.1016/0092-8674(82)90135-0. PMID 6186384.
- Proudfoot, N. J.; Brownlee, G. G. (1976). "3′ Non-coding region sequences in eukaryotic messenger RNA". Nature 263 (5574): 211–214. doi:10.1038/263211a0. PMID 822353.
- Choo, K. H.; Gould, K. G.; Rees, D. J. G.; Brownlee, G. G. (1982). "Molecular cloning of the gene for human anti-haemophilic factor IX". Nature 299 (5879): 178–180. doi:10.1038/299178a0. PMID 6287289.
- Anson, D. S.; Austen, D. E. G.; Brownlee, G. G. (1985). "Expression of active human clotting factor IX from recombinant DNA clones in mammalian cells". Nature 315 (6021): 683–685. doi:10.1038/315683a0. PMID 2989700.
- "Fred Sanger, Double Nobel Laureate: A Biography". Cambridge UP. Retrieved 22 August 2014.