SR 388 begins at an intersection with SR 223 and Harlem–Grovetown Road in Grovetown. Here, SR 388 is known as Wrightsboro Road, a major road farther to the east in Augusta. SR 223 north of here is also known as Wrightsboro Road, but is known as West Robinson Avenue south of here. SR 388 travels to the east for about 0.8 miles (1.3 km) until it meets the Katherine Street/Wrightsboro Road/Horizon South Parkway intersection. At this intersection, SR 388 turns left onto Horizon South Parkway, while Wrightsboro Road continues to the east toward Augusta. North of that intersection, the road passes a campus of Augusta Technical College, before it has an interchange with Interstate 20 (I-20). Here, the roadway becomes known as Lewiston Road. A short distance later, it meets its northern terminus, an intersection with SR 232 (Columbia Road), where the roadway continues as Hereford Farm Road, and leads to Evans. SR 388 serves to connect SR 223 and SR 232, which run parallel to each other on opposite sides of I-20 in southern Columbia County. It and Hereford Farm Road also serve to connect Grovetown and Evans.
SR 388 is not part of the National Highway System, a system of routes determined to be the most important for the nation's economy, mobility and defense.