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Rho GTPase activating protein 26
Protein ARHGAP26 PDB 1f7c.png
PDB rendering based on 1f7c.
Available structures
PDB Ortholog search: PDBe, RCSB
External IDs OMIM605370 MGI1918552 HomoloGene36349 GeneCards: ARHGAP26 Gene
RNA expression pattern
PBB GE ARHGAP26 205068 s at tn.png
PBB GE ARHGAP26 205069 s at tn.png
PBB GE ARHGAP26 215955 x at tn.png
More reference expression data
Species Human Mouse
Entrez 23092 71302
Ensembl ENSG00000145819 ENSMUSG00000036452
UniProt Q9UNA1 Q6ZQ82
RefSeq (mRNA) NM_001135608 NM_175164
RefSeq (protein) NP_001129080 NP_780373
Location (UCSC) Chr 5:
142.15 – 142.61 Mb
Chr 18:
38.6 – 39.38 Mb
PubMed search [1] [2]

Rho GTPase activating protein 26 (ARHGAP26) also known as GTPase Regulator Associated with Focal Adhesion Kinase (GRAF) is a protein that in humans is encoded by the ARHGAP26 gene.[1][2][3]


GRAF is a multidomain protein that is necessary for the CLIC/GEEC endocytic pathway,[4] the most prevalent clathrin-independent endocytic pathway discovered to date. By virtue of an N-terminal BAR domain, GRAF1 sculpts the endocytic membranes of this pathway into 40 nm diameter tubules and vesicles that allow uptake of extracellular fluid, GPI-linked proteins and certain bacterial exotoxins into cells. The role of dynamin in the CLIC/GEEC pathway is controversial, but GRAF1 interacts strongly with this protein and acute inhibition of dynamin action abrogates CLIC/GEEC endocytosis. There are several members of the GRAF family of proteins, including GRAF2, GRAF3, and oligophrenin, all of which likely playing similar roles during clathrin-independent endocytic events. Mutations of both GRAF1 and oligophrenin are strongly implicated in causing human disease (leukaemia and mental retardation, respectively).


ARHGAP26 has been shown to interact with PKN3.[5]


  1. ^ "Entrez Gene: ARHGAP26 Rho GTPase activating protein 26". 
  2. ^ Hildebrand JD, Taylor JM, Parsons JT (June 1996). "An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase". Mol. Cell. Biol. 16 (6): 3169–78. PMC 231310. PMID 8649427. 
  3. ^ Taylor JM, Macklem MM, Parsons JT (January 1999). "Cytoskeletal changes induced by GRAF, the GTPase regulator associated with focal adhesion kinase, are mediated by Rho". J. Cell. Sci. 112 ( Pt 2): 231–42. PMID 9858476. 
  4. ^ Lundmark R, Doherty GJ, Howes MT, Cortese K, Vallis Y, Parton RG, McMahon HT (November 2008). "The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic Pathway". Curr. Biol. 18 (22): 1802–8. doi:10.1016/j.cub.2008.10.044. PMC 2726289. PMID 19036340. 
  5. ^ Shibata H, Oishi K, Yamagiwa A, Matsumoto M, Mukai H, Ono Y (2001). "PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family". J. Biochem. 130 (1): 23–31. doi:10.1093/oxfordjournals.jbchem.a002958. PMID 11432776. 

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