HUS1

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HUS1 checkpoint homolog (S. pombe)
Protein HUS1 PDB 3A1J.png
Rendering based on PDB 3A1J.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols HUS1 ; hHUS1
External IDs OMIM603760 MGI1277962 HomoloGene37932 GeneCards: HUS1 Gene
RNA expression pattern
PBB GE HUS1 204884 s at tn.png
PBB GE HUS1 204883 s at tn.png
PBB GE HUS1 217618 x at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 3364 15574
Ensembl ENSG00000136273 ENSMUSG00000020413
UniProt O60921 Q8BQY8
RefSeq (mRNA) NM_004507 NM_008316
RefSeq (protein) NP_004498 NP_032342
Location (UCSC) Chr 7:
47.74 – 48.02 Mb
Chr 11:
8.99 – 9.01 Mb
PubMed search [1] [2]

Checkpoint protein HUS1 is a protein that in humans is encoded by the HUS1 gene.[1][2]

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event.[3]

Interactions[edit]

HUS1 has been shown to interact with RAD9A,[4][5][6][7] RAD17,[8][9] HDAC1,[10] RAD1 homolog[5][11] and PCNA.[12]

References[edit]

  1. ^ Dean FB, Lian L, O'Donnell M (February 1999). "cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster". Genomics 54 (3): 424–36. doi:10.1006/geno.1998.5587. PMID 9878245. 
  2. ^ Kostrub CF, Knudsen K, Subramani S, Enoch T (June 1998). "Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage". EMBO J 17 (7): 2055–66. doi:10.1093/emboj/17.7.2055. PMC 1170550. PMID 9524127. 
  3. ^ "Entrez Gene: HUS1 HUS1 checkpoint homolog (S. pombe)". 
  4. ^ Dufault, Vanessa M; Oestreich Andrea J; Vroman Benjamin T; Karnitz Larry M (December 2003). "Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene". Genomics (United States) 82 (6): 644–51. doi:10.1016/S0888-7543(03)00200-3. ISSN 0888-7543. PMID 14611806. 
  5. ^ a b Volkmer, E; Karnitz L M (January 1999). "Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex". J. Biol. Chem. (UNITED STATES) 274 (2): 567–70. doi:10.1074/jbc.274.2.567. ISSN 0021-9258. PMID 9872989. 
  6. ^ Griffith, Jack D; Lindsey-Boltz Laura A, Sancar Aziz (May 2002). "Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy". J. Biol. Chem. (United States) 277 (18): 15233–6. doi:10.1074/jbc.C200129200. ISSN 0021-9258. PMID 11907025. 
  7. ^ Hirai, Itaru; Wang Hong-Gang (July 2002). "A role of the C-terminal region of human Rad9 (hRad9) in nuclear transport of the hRad9 checkpoint complex". J. Biol. Chem. (United States) 277 (28): 25722–7. doi:10.1074/jbc.M203079200. ISSN 0021-9258. PMID 11994305. 
  8. ^ Bermudez, Vladimir P; Lindsey-Boltz Laura A, Cesare Anthony J, Maniwa Yoshimasa, Griffith Jack D, Hurwitz Jerard, Sancar Aziz (February 2003). "Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro". Proc. Natl. Acad. Sci. U.S.A. (United States) 100 (4): 1633–8. doi:10.1073/pnas.0437927100. ISSN 0027-8424. PMC 149884. PMID 12578958. 
  9. ^ Rauen, M; Burtelow M A; Dufault V M; Karnitz L M (September 2000). "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9". J. Biol. Chem. (UNITED STATES) 275 (38): 29767–71. doi:10.1074/jbc.M005782200. ISSN 0021-9258. PMID 10884395. 
  10. ^ Cai, R L; Yan-Neale Y; Cueto M A; Xu H; Cohen D (September 2000). "HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins". J. Biol. Chem. (UNITED STATES) 275 (36): 27909–16. doi:10.1074/jbc.M000168200. ISSN 0021-9258. PMID 10846170. 
  11. ^ Hang, Haiying; Zhang Yuzhu; Dunbrack Roland L; Wang Cuidong; Lieberman Howard B (April 2002). "Identification and characterization of a paralog of human cell cycle checkpoint gene HUS1". Genomics (United States) 79 (4): 487–92. doi:10.1006/geno.2002.6737. ISSN 0888-7543. PMID 11944979. 
  12. ^ Komatsu, K; Wharton W; Hang H; Wu C; Singh S; Lieberman H B; Pledger W J; Wang H G (November 2000). "PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition". Oncogene (ENGLAND) 19 (46): 5291–7. doi:10.1038/sj.onc.1203901. ISSN 0950-9232. PMID 11077446. 

Further reading[edit]