Haplodiploidy is a sex-determination system in which males develop from unfertilized eggs and are haploid, and females develop from fertilized eggs and are diploid. Haplodiploidy is sometimes called arrhenotoky.
Haplodiploidy determines the sex in all members of the insect order Hymenoptera (bees, ants, and wasps),p408 Coccidae, and the Thysanoptera ('thrips'). The system also occurs sporadically in some spider mites, Homoptera, Coleoptera (bark beetles), and rotifers.
In this system, sex is determined by the number of sets of chromosomes an individual receives. An offspring formed from the union of a sperm and an egg develops as a female, and an unfertilized egg develops as a male. This means that the males have half the number of chromosomes that a female has, and are haploid.
The haplodiploid sex-determination system has a number of peculiarities; for example, a male has no father and cannot have sons, but he has a grandfather and can have grandsons. And, more to the point, the relatedness between worker bees (diploid females) in a hive or nest is 0.75. This means the workers are significantly more closely related than siblings in other sex determination systems. It is this point which drives the kin selection theory of how eusociality evolved.p465 Whether haplodiploidy did in fact pave the way for the evolution of eusociality is still a matter of debate.
Several models have been proposed for the genetic mechanisms of haplodiploid sex-determination. The model most commonly referred to is the complementary allele model. According to this model, if an individual is heterozygous for a certain locus, it develops into a female, whereas hemizygous and homozygous individuals develop into males. In other words, diploid offspring develop from fertilized eggs, and are normally female, while haploid offspring develop into males from unfertilized eggs. Diploid males would be infertile, as their cells would not undergo meiosis to form sperm. Therefore the sperm would be diploid, which means that their offspring would be triploid. Since hymenopteran mother and sons share the same genes, they may be especially sensitive to inbreeding: Inbreeding reduces the number of different sex alleles present in a population, hence increasing the occurrence of diploid males.
After mating, each fertile Hymenopteran female stores sperm in an internal sac called the spermatheca. The mated female controls the release of stored sperm from within the organ: If she releases sperm as an egg passes down her oviduct, the egg is fertilized. Social bees, wasps, and ants can modify sex ratios within colonies which maximizes relatedness among members and generates a workforce appropriate to surrounding conditions. In other solitary hymenopterans, the females lay unfertilized male eggs on poorer food sources while laying the fertilized female eggs on better food sources, possibly because the fitness of females will be more adversely affected by shortages in their early life. Sex ratio manipulation is also practiced by haplodiploid ambrosia beetles, who lay more male eggs when the chances for males to disperse and mate with females in different sites is more.
Sex-determination in honey bees
In honeybees the drones (males) are entirely derived from the queen, their mother. The diploid queen has 32 chromosomes and the haploid drones have 16 chromosomes. Drones produce sperm cells that contain their entire genome, so the sperm are all genetically identical except for mutations. The genetic makeup of the female worker bees is half derived from the mother, and half from the father, but the male bees' genetic makeup is entirely derived from the mother. Thus, if a queen bee mates with only one drone, any two of her daughters will share, on average, 3/4 of their genes. The diploid queen's genome is recombined for her daughters, but the haploid father's genome is inherited by his daughters "as is".
There are rare instances of diploid drone larvae. This phenomena usually arises when there is more than two generations of brother-sister mating. Sex determination in honey bees is initially due to a single locus, called the complementary sex determiner (csd) gene. In developing bees, if the conditions are that the individual is heterozygous for the csd gene, they will develop into females. If the conditions are so that the individual is hemizygous or homozygous for the csd gene, they will develop into males. The instances were the individual is homozygous at this gene are the instances of diploid males. Diploid males do not survive to adulthood, as the nurse worker bees will cannibalize the diploid males upon hatching.
While workers can lay unfertilized eggs that become their sons, haplodiploid sex-determination system increases the individual's fitness due to indirect selection. Since the worker is more related to the queen's daughters (her sisters) than to her own offspring, helping the queen's offspring to survive aids the spread of the same genes that the worker possesses more efficiently than direct reproduction.  Batches of worker bees are short lived and are constantly being replaced by the next batch, so this kin selection is possibly a strategy to ensure the proper working of the hive. However, since queens usually mate with a dozen drones or more, not all workers are full sisters. Due to the separate storage of drone sperm, a specific batch of brood may be more closely related than a specific batch of brood laid at a later date.
Relatedness ratios in haplodiploidy
Relatedness is used to calculate the strength of kin selection (via Hamilton's rule). The haplodiploidy hypothesis states that the unusual 3/4 relatedness coefficient amongst full haplodiploid sisters is responsible for the frequency of evolution of eusocial behavior in hymenoptera.
In normal sexual reproduction the father has two sets of chromosomes, and crossing over takes place between the chromatids of each pair during the meiosis which produces the sperm. Therefore, the sperms are not identical, because in each chromosome of a pair there will be different alleles at many of the loci. But when the father is haploid all the sperm will be identical (except for a small number where gene mutations have taken place in the germ line). So, as long as all the female queen mates only with one male, all the female offspring will inherit the male's chromosome 100% intact. In fact in hymenoptera, the males almost all produce enough sperm to last the female for a whole lifetime from a single mating event.
Relatedness coefficients in haplodiploid organisms are as follows.
|Sex||Daughter||Son||Mother||Father||Full Sister||Full Brother|
These unique relatedness ratios were thought to be the main factor in the evolution of eusociality in many hymenopterans.
- Green-beard effect
- Sex-determination system
- Sexual differentiation
- Worker policing
- X chromosome
- Y chromosome
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