The Harrisburg Line is a railroad line owned and operated by the Norfolk Southern Railway (NS) in the U.S. state of Pennsylvania. The line runs from Philadelphia west to Harrisburg along the former Reading Company main line. The line is part of the NS Harrisburg Division.
At its east end, near the Falls of the Schuylkill, the Harrisburg Line junctions with CSX Transportation's Trenton Subdivision. Its west end is at a junction with the Pittsburgh Line, the Lurgan Branch, the Royalton Branch, and Amtrak's Philadelphia to Harrisburg Main Line. The line junctions the Morrisville Line (indirectly) at Norristown and the Reading Line near Reading.
The Philadelphia and Reading Rail Road opened in 1839, including the current Harrisburg Line east of Reading. The line from Reading to Harrisburg was opened by the Lebanon Valley Railroad in 1858. Through leases, mergers, and takeovers, the line eventually became part of Conrail in 1976. In the 1999 breakup of Conrail, the line was assigned to Norfolk Southern.